College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan 625014, China.
Biol Trace Elem Res. 2010 Dec;138(1-3):173-80. doi: 10.1007/s12011-009-8599-z. Epub 2010 Jan 20.
The experiment was conducted with the objective of evaluating the effect of dietary high fluorine (F) on cell cycle and apoptosis of kidney in chickens by the methods of flow cytometry. Three hundred 1-day-old Avian broilers were divided into four groups and fed on control diet (F 23 mg/kg) and high F diets (400 mg/kg, high F group I; 800 mg/kg, high F group II; 1,200 mg/kg, high F group III) for 6 weeks. As tested by flow cytometry, the percentage of renal cell apoptosis was increased with increasing of dietary F, and it obviously rose in three high F groups when compared with that of control group. Renal cells in G(0)/G(1) phase were much higher, and renal cells in S phase, G(2)+M phase, and proliferation index value were much lower in high F groups I, II, and III than in control group. The results showed that excess dietary F in the range of 400-1,200 mg/kg caused G(0)/G(1) arrest and increased cellular apoptosis in the kidney, which might finally interfere with the excretion and retention of fluoride in chickens.
该实验旨在通过流式细胞术方法研究饲粮高氟（F）对鸡肾脏细胞周期和细胞凋亡的影响。选用 300 只 1 日龄爱拔益加（AA）肉鸡，随机分为 4 个组，分别饲喂基础饲粮（F 含量 23mg/kg）和高 F 饲粮（400mg/kg，高 F Ⅰ组；800mg/kg，高 F Ⅱ组；1200mg/kg，高 F Ⅲ组）6 周。流式细胞术检测结果表明，随着饲粮 F 水平的升高，鸡肾脏细胞凋亡率逐渐升高，且高 F Ⅰ、Ⅱ、Ⅲ组均极显著高于对照组（P＜0.01）。高 F Ⅰ、Ⅱ、Ⅲ组 G0/G1 期细胞比例极显著高于对照组（P＜0.01），S 期、G2/M 期细胞比例及增殖指数极显著低于对照组（P＜0.01）。说明 400～1200mg/kg 的饲粮 F 可导致 G0/G1 期阻滞和细胞凋亡增加，进而可能干扰氟在鸡体内的排泄和蓄积。
