Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, Sichuan, 625014, China.
Biol Trace Elem Res. 2011 Dec;143(3):1508-15. doi: 10.1007/s12011-011-8993-1. Epub 2011 Feb 18.
The objective of this study was to clarify the effects of dietary vanadium on cell cycle and apoptosis of liver in broilers. Four hundred and twenty one-day-old avian broilers were divided into six groups and fed on a corn-soybean basal diet as control diet or the same diet amended to contain 5, 15, 30, 45, and 60 mg/kg vanadium supplied as ammonium metavanadate for 42 days. As tested by flow cytometry, hepatocytes in G (0)/G (1) phase were significantly increased in number in 45 and 60 mg/kg groups, and hepatocytes in S, G (2) + M phases in 45 and 60 mg/kg groups and the proliferation index of hepatocytes in 30, 45, and 60 mg/kg were markedly decreased when compared with those of control group. At the same time, the percentage of hepatocyte apoptosis was markedly increased in both 45 and 60 mg/kg groups. The results showed that dietary vanadium in the range of 45 ∼ 60 mg/kg caused cell cycle arrest and apoptosis of hepatocytes in broilers.
本研究旨在阐明日粮钒对肉鸡肝脏细胞周期和细胞凋亡的影响。将 421 日龄禽肉鸡分为 6 组，分别饲喂玉米-豆粕基础日粮（对照组）或添加 5、15、30、45 和 60mg/kg 五氧化二钒（以偏钒酸铵形式添加）的相同日粮，试验期 42 天。流式细胞术检测结果表明，与对照组相比，45 和 60mg/kg 组 G0/G1 期肝细胞数量显著增加，45 和 60mg/kg 组 S 期、G2/M 期肝细胞数量及 30、45 和 60mg/kg 组的肝细胞增殖指数显著降低。同时，45 和 60mg/kg 组的肝细胞凋亡率也显著增加。结果表明，日粮钒在 45∼60mg/kg 范围内可引起肉鸡肝细胞周期阻滞和凋亡。
