Licciulli Silvia, Luise Chiara, Zanardi Andrea, Giorgetti Luca, Viale Giuseppe, Lanfrancone Luisa, Carbone Roberta, Alcalay Myriam
Department of Experimental Oncology, Istituto Europeo di Oncologia, Via Adamello 16, 20139, Milan, Italy.
BMC Cell Biol. 2010 Jan 20;11:5. doi: 10.1186/1471-2121-11-5.
Pirin (PIR) is a highly conserved nuclear protein originally isolated as an interactor of NFI/CTF1 transcription/replication factor. It is a member of the functionally diverse cupin superfamily and its activity has been linked to different biological and molecular processes, such as regulation of transcription, apoptosis, stress response and enzymatic processes. Although its precise role in these functions has not yet been defined, PIR expression is known to be deregulated in several human malignancies.
We performed immunohistochemical analysis of PIR expression in primary samples from normal human tissues and tumors and identified a dislocation of PIR to the cytoplasm in a subset of melanomas, and a positive correlation between cytoplasmic PIR levels and melanoma progression. PIR localization was subsequently analyzed in vitro in melanoma cell lines through a high content immunofluorescence based approach (ImmunoCell-Array).
The high consistency between in vivo and in vitro results obtained by immunohistochemistry and ImmunoCell-Array provides a validation of the potential of ImmunoCell-Array technology for the rapid screening of putative biological markers, and suggests that cytoplasmic localization of PIR may represent a characteristic of melanoma progression.
吡啉（PIR）是一种高度保守的核蛋白，最初作为NFI/CTF1转录/复制因子的相互作用蛋白被分离出来。它是功能多样的cupin超家族的成员，其活性与不同的生物学和分子过程相关，如转录调控、细胞凋亡、应激反应和酶促过程。尽管其在这些功能中的精确作用尚未明确，但已知PIR表达在几种人类恶性肿瘤中失调。
我们对来自正常人体组织和肿瘤的原发性样本进行了PIR表达的免疫组织化学分析，发现在一部分黑色素瘤中PIR向细胞质移位，且细胞质PIR水平与黑色素瘤进展呈正相关。随后通过基于高内涵免疫荧光的方法（免疫细胞阵列）在黑色素瘤细胞系中进行了体外PIR定位分析。
免疫组织化学和免疫细胞阵列在体内和体外获得的结果高度一致，验证了免疫细胞阵列技术在快速筛选潜在生物标志物方面的潜力，并表明PIR的细胞质定位可能代表黑色素瘤进展的一个特征。
