Carpenter Anne E, Jones Thouis R, Lamprecht Michael R, Clarke Colin, Kang In Han, Friman Ola, Guertin David A, Chang Joo Han, Lindquist Robert A, Moffat Jason, Golland Polina, Sabatini David M
Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
Genome Biol. 2006;7(10):R100. doi: 10.1186/gb-2006-7-10-r100. Epub 2006 Oct 31.
Biologists can now prepare and image thousands of samples per day using automation, enabling chemical screens and functional genomics (for example, using RNA interference). Here we describe the first free, open-source system designed for flexible, high-throughput cell image analysis, CellProfiler. CellProfiler can address a variety of biological questions quantitatively, including standard assays (for example, cell count, size, per-cell protein levels) and complex morphological assays (for example, cell/organelle shape or subcellular patterns of DNA or protein staining).
如今，生物学家借助自动化技术每天能够制备并成像数千个样本，从而实现化学筛选和功能基因组学研究（例如，使用RNA干扰技术）。在此，我们介绍首个专为灵活的高通量细胞图像分析而设计的免费开源系统——细胞图像分析软件（CellProfiler）。CellProfiler能够定量解决各种生物学问题，包括标准检测（例如，细胞计数、大小、细胞内蛋白质水平）以及复杂的形态学检测（例如，细胞/细胞器形状或DNA或蛋白质染色的亚细胞模式）。
Zotero 插件
