M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences, Moscow Region 142782, Russia.
J Gen Virol. 2010 May;91(Pt 5):1239-44. doi: 10.1099/vir.0.017723-0. Epub 2010 Jan 20.
Both entero- and cardioviruses have been shown to suppress host mRNA synthesis. Enteroviruses are also known to inhibit the activity of rRNA genes, whereas this ability of cardioviruses is under debate. This study reported that mengovirus (a cardiovirus) suppressed rRNA synthesis but less efficiently than poliovirus (an enterovirus). In contrast to poliovirus infection, the incorporation of BrUTP, fluorouridine and [14C]uridine in rRNA precursors was observed even during the late stages of mengovirus infection, although at a significantly reduced level. The cleavage of TATA-binding protein, considered to be one of the central events in poliovirus-induced transcription shutoff, was not detected in mengovirus-infected cells, indicating a difference in the mechanisms of host RNA synthesis inhibition caused by these viruses. The results also showed that functional leader protein is redundant for the suppression of host RNA synthesis by cardiovirus.
肠病毒和心病毒已被证实可抑制宿主 mRNA 的合成。肠病毒还被证实可以抑制 rRNA 基因的活性，而心病毒的这种能力仍存在争议。本研究报道称，细小病毒（一种心病毒）抑制 rRNA 的合成，但效率低于脊髓灰质炎病毒（一种肠病毒）。与脊髓灰质炎病毒感染不同，即使在细小病毒感染的晚期，也观察到 BrUTP、氟尿苷和[14C]尿苷掺入 rRNA 前体，尽管水平显著降低。TATA 结合蛋白的切割被认为是脊髓灰质炎病毒诱导的转录关闭的中心事件之一，但在细小病毒感染的细胞中未检测到，这表明这些病毒引起的宿主 RNA 合成抑制的机制存在差异。研究结果还表明，功能性的 5' 端非翻译区对于心病毒抑制宿主 RNA 合成是冗余的。
