Bienz K, Egger D, Rasser Y, Loeffler H
Intervirology. 1978;10(4):209-20. doi: 10.1159/000148984.
Patterns of cellular RNA synthesis during poliovirus (types 1,2 and 3) and mengovirus infections in HeLa, HEp-2, Vero and L-cells were investigated by means of high-resolution autoradiography, kinetics of 3H-uridine incorporation, and sucrose gradient centrifugation. Only mengovirus-infected L-cells showed an early and exponential inhibition of hnRNA and rRNA synthesis. The other cell lines tested, whether infected with polio- or mengovirus, exhibited a slowly and linearly decreasing cellular RNA synthesis, with hnRNA production suppressed completely, although somewhat later than in L-cells, and rRNA synthesis continuing at a gradually reduced rate during the entire viral growth cycle. Locations of cleavage steps of rRNA precursor molecules are discussed.
通过高分辨率放射自显影、³H-尿苷掺入动力学和蔗糖梯度离心法，研究了脊髓灰质炎病毒（1型、2型和3型）和脑心肌炎病毒感染HeLa、HEp-2、Vero和L细胞期间的细胞RNA合成模式。只有感染脑心肌炎病毒的L细胞显示出hnRNA和rRNA合成的早期指数抑制。其他受试细胞系，无论感染脊髓灰质炎病毒还是脑心肌炎病毒，细胞RNA合成均呈缓慢线性下降，hnRNA产生完全受到抑制，尽管比L细胞稍晚，且rRNA合成在整个病毒生长周期中以逐渐降低的速率持续进行。讨论了rRNA前体分子切割步骤的位置。
