Cai L, Kong F, Toi C, van Hal S, Gilbert G L
Department of Dermatology, Peking University People's Hospital, Beijing 100044, People's Republic of China.
Int J STD AIDS. 2010 Feb;21(2):101-4. doi: 10.1258/ijsa.2009.009013.
Lymphogranuloma venereum (LGV) is a sexually transmitted infection caused by Chlamydia trachomatis serovars L1, L2 and L3. Consequently, more specific and sensitive detection methods that are rapid and inexpensive are necessary to differentiate between C. trachomatis serovars. The purpose of this study was to identify and differentiate LGV-related C. trachomatis serovars from rectal swabs using high-resolution melting analysis (HRMA) and multiplex allele-specific polymerase chain reaction (MAS-PCR). Fifteen clinical samples from patients in Sydney were first screened and confirmed as C. trachomatis by using the COBAS AMPLICOR PCR analyser. The same samples were assayed for C. trachomatis and LGV by HRMA and MAS-PCR of the polymorphic membrane protein H (pmpH) gene. Both methods indicated that two of 15 samples were serovar L2 and the remainder (13/15) other C. trachomatis serovars. Both HRMA and MAS-PCR are inexpensive, rapid, easy methods that are useful tools for the identification of LGV in clinical and research laboratories.
性病性淋巴肉芽肿（LGV）是由沙眼衣原体L1、L2和L3血清型引起的一种性传播感染。因此，需要更特异、灵敏、快速且廉价的检测方法来区分沙眼衣原体的不同血清型。本研究的目的是使用高分辨率熔解分析（HRMA）和多重等位基因特异性聚合酶链反应（MAS-PCR）从直肠拭子中鉴定并区分与LGV相关的沙眼衣原体血清型。首先使用COBAS AMPLICOR PCR分析仪对来自悉尼患者的15份临床样本进行筛查，并确认为沙眼衣原体感染。通过对多态性膜蛋白H（pmpH）基因进行HRMA和MAS-PCR，对相同样本检测沙眼衣原体和LGV。两种方法均表明，15份样本中有2份为L2血清型，其余13份为其他沙眼衣原体血清型。HRMA和MAS-PCR都是廉价、快速、简便的方法，是临床和研究实验室鉴定LGV的有用工具。
