Chen Cheng-Yen, Chi Kai-Hua, Alexander Sarah, Martin Iona M C, Liu Hsi, Ison Cathy A, Ballard Ronald C
Division of STD Prevention, National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Sex Transm Dis. 2007 Jul;34(7):451-5. doi: 10.1097/01.olq.0000245957.02939.ea.
The objectives of this study were to evaluate the use of a real-time multiplex polymerase chain reaction (M-PCR) assay to differentiate between trachoma and lymphogranuloma venereum (LGV) biovars of Chlamydia trachomatis and to validate its performance with the conventional genotyping method.
Swab specimens from 115 patients with anorectal symptoms or syndromes associated with LGV were tested by a real-time M-PCR assay and the results compared with the PCR-based restriction fragment length polymorphism analysis of the major outer membrane protein gene (omp1).
A high agreement of 96.5% (111 of 115 specimens) was found between the real-time M-PCR testing and the standard genotyping method for the detection of C. trachomatis DNA (kappa value, 0.945, P <0.00001). Both methods identified 53 LGV, 32 non-LGV C. trachomatis, and 26 negative specimens.
The real-time M-PCR assay simultaneously detects and differentiates LGV from non-LGV strains using swab specimens. This assay offers a relatively rapid and sensitive alternative for the diagnosis of LGV infection and is a useful tool for screening and for outbreak investigations.
本研究的目的是评估使用实时多重聚合酶链反应（M-PCR）检测法区分沙眼衣原体的沙眼生物变种和性病淋巴肉芽肿（LGV）生物变种，并通过传统基因分型方法验证其性能。
对115例有与LGV相关的肛门直肠症状或综合征的患者的拭子标本进行实时M-PCR检测，并将结果与基于聚合酶链反应的主要外膜蛋白基因（omp1）限制性片段长度多态性分析结果进行比较。
实时M-PCR检测与检测沙眼衣原体DNA的标准基因分型方法之间的一致性高达96.5%（115个标本中的111个）（kappa值为0.945，P<0.00001）。两种方法均鉴定出53例LGV、32例非LGV沙眼衣原体和26例阴性标本。
实时M-PCR检测法可使用拭子标本同时检测LGV和非LGV菌株并进行区分。该检测法为LGV感染的诊断提供了一种相对快速且灵敏的替代方法，是筛查和疫情调查的有用工具。
