采用定量高分辨率熔解分析（HRMA）快速检测沙眼衣原体淋球菌血清型。
Rapid determination of lymphogranuloma venereum serovars of Chlamydia trachomatis by quantitative high-resolution melt analysis (HRMA).
机构信息
Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Melbourne, Australia.
出版信息
J Clin Microbiol. 2012 Nov;50(11):3751-3. doi: 10.1128/JCM.01670-12. Epub 2012 Aug 29.
Abstract
A quantitative high-resolution melt analysis assay was developed to differentiate lymphogranuloma venereum-causing serovars of Chlamydia trachomatis (L1 to L3) from other C. trachomatis serovars (D to K). The detection limit of this assay is approximately 10 copies per reaction, comparable to the limits of other quantitative-PCR-based methods.
摘要
建立了一种定量高分辨率熔解分析检测方法，用于区分引起淋菌性肉芽肿的沙眼衣原体血清型（L1 至 L3）与其他沙眼衣原体血清型（D 至 K）。该检测方法的检测限约为每个反应 10 个拷贝，与其他基于定量 PCR 的方法相当。
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