Tan Geok Chin, Norlatiffah Sydee, Sharifah N Akmal, Razmin Ghazali, Shiran M Sidik, Hatta A Zailani, Paul-Ng H Oon
Department of Pathology and Obstetrics, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Indian J Pathol Microbiol. 2010 Jan-Mar;53(1):1-6. doi: 10.4103/0377-4929.59173.
Cervical cancer is the second most common cancer affecting Malaysian women. Despite the implementation of pap smear screening, many women are still diagnosed only in the advanced stage of cervical cancer. This could partly be due to failure of detection of its precursor lesions; hence the need to search for novel biomarkers to assist in the screening and diagnosis of cervical neoplasia. This study aims to determine the expression of p16INK4A and survivin as possible predictive biomarkers in cervical squamous neoplasm.
This is a retrospective study on 201 cases of cervical neoplasm comprising of 129 cervical intraepithelial neoplasia (CIN) and 72 squamous cell carcinoma (SCC). All samples were evaluated by two independent observers using p16INK4A and survivin monoclonal antibodies. The p16 INK4A expression was graded as negative, focal and diffuse positivity. The intensity for survivin expression was graded as weak, moderate and intense.
It is seen that p16 INK4A expression in CIN 1, CIN 2 and CIN 3 were 25.4%, 42.9% and 95.9% respectively. Majority of SCC (98.6%) showed p16 INK4A expression. Survivin expressions in CIN 1, CIN 2, CIN 3 and SCC were 56.7%, 33.4%, 87.5% and 98.6%. There was a linear relationship between increasing grade of CIN and p16 INK4A expressions.
Our study showed that p16 INK4A expressions correlate well with the increasing grade of CIN. Although survivin does not correlate well to the increasing grade of CIN, it could be useful in differentiating CIN 3 from SCC.
宫颈癌是影响马来西亚女性的第二大常见癌症。尽管实施了巴氏涂片筛查，但许多女性仍在宫颈癌晚期才被诊断出来。这部分可能是由于其前驱病变检测失败；因此需要寻找新的生物标志物来辅助宫颈癌变的筛查和诊断。本研究旨在确定p16INK4A和生存素作为宫颈鳞状肿瘤可能的预测生物标志物的表达情况。
这是一项对201例宫颈肿瘤的回顾性研究，包括129例宫颈上皮内瘤变（CIN）和72例鳞状细胞癌（SCC）。所有样本由两名独立观察者使用p16INK4A和生存素单克隆抗体进行评估。p16INK4A表达分为阴性、局灶性和弥漫性阳性。生存素表达强度分为弱、中、强。
可见CIN 1、CIN 2和CIN 3中p16INK4A表达分别为25.4%、42.9%和95.9%。大多数SCC（98.6%）显示p16INK4A表达。CIN 1、CIN 2、CIN 3和SCC中生存素表达分别为56.7%、33.4%、87.5%和98.6%。CIN分级增加与p16INK4A表达之间存在线性关系。
我们的研究表明，p16INK4A表达与CIN分级增加密切相关。虽然生存素与CIN分级增加的相关性不佳，但它可能有助于将CIN 3与SCC区分开来。
