Zhang Yongsheng, Guo Liangsheng, Xing Pengfei, Chen Yuanyuan, Li Feng, Zhu Weipei, Lu Xueguan
Department of Pathology, The Second Affiliated Hospital of Soochow University Suzhou, China.
Department of Gynecology, The Second Affiliated Hospital of Soochow University Suzhou, China.
Int J Clin Exp Pathol. 2014 Dec 1;7(12):8911-6. eCollection 2014.
To investigate the expression of p15(INK4b), p16(INK4a) and p21(Waf1/Cip1) in specimens from cases of normal cervical epithelium (NCE), cervical intraepithelial neoplasia (CIN) and squamous cell carcinoma (SCC), and to evaluate whether there is evidence implicating oncogene-induced senescence (OIS) in cervical squamous cell cancer development.
The immunohistochemical expression of p15(INK4b), p16(INK4a) and p21(Waf1/Cip1) were investigated in formalin-fixed paraffin-embedded specimens from 19 NCE, 51 CIN and 21 SCC cases, respectively. Comparisons among different groups for each marker were performed with Chi-square test.
The expression of p15(INK4b), p16(INK4a) and p21(Waf1/Cip1) were significantly higher in both CIN and SCC compared to NCE. Furthermore, the expression of p15(INK4b) and p21(Waf1/Cip1) was significantly higher in CIN П compared to CIN І, and these expressions were statistically higher in CIN Ш compared to CIN П, respectively. The p16(INK4a) expression was significantly higher in CIN Ш compared to CIN І.
The results suggested that the senescence programs mediated by p15(INK4b), p16(INK4a) and p21(Waf1/Cip1) were activated during the stage of CIN and SCC, and demonstrated that senescence may play important role in preventing from NCE to SCC.
研究p15（INK4b）、p16（INK4a）和p21（Waf1/Cip1）在正常宫颈上皮（NCE）、宫颈上皮内瘤变（CIN）和鳞状细胞癌（SCC）病例标本中的表达情况，并评估是否有证据表明致癌基因诱导的衰老（OIS）参与宫颈鳞状细胞癌的发生发展。
分别对19例NCE、51例CIN和21例SCC病例的福尔马林固定石蜡包埋标本进行p15（INK4b）、p16（INK4a）和p21（Waf1/Cip1）的免疫组化表达研究。对每个标志物在不同组间进行卡方检验比较。
与NCE相比，CIN和SCC中p15（INK4b）、p16（INK4a）和p21（Waf1/Cip1）的表达均显著升高。此外，与CINⅠ相比，CINⅡ中p15（INK4b）和p21（Waf1/Cip1）的表达显著升高，且与CINⅡ相比，CINⅢ中这些表达在统计学上更高。与CINⅠ相比，CINⅢ中p16（INK4a）的表达显著升高。
结果表明，由p15（INK4b）、p16（INK4a）和p21（Waf1/Cip1）介导的衰老程序在CIN和SCC阶段被激活，并表明衰老可能在预防从NCE发展为SCC中起重要作用。
