碳酸酐酶 I、II 和 VI、血浆、红细胞和唾液中的锌和铜在重复经颅磁刺激后增加。

Carbonic anhydrase I, II, and VI, blood plasma, erythrocyte and saliva zinc and copper increase after repetitive transcranial magnetic stimulation.

机构信息

Taste and Smell Clinic, Washington, District of Columbia 20016, USA.

出版信息

Am J Med Sci. 2010 Mar;339(3):249-57. doi: 10.1097/MAJ.0b013e3181cda0e3.

DOI:10.1097/MAJ.0b013e3181cda0e3

PMID:20090508

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2928063/

Abstract

INTRODUCTION

Repetitive transcranial magnetic stimulation (rTMS) has been used to treat symptoms from many disorders; biochemical changes occurred with this treatment. Preliminary studies with rTMS in patients with taste and smell dysfunction improved sensory function and increased salivary carbonic anhydrase (CA) VI and erythrocyte CA I, II. To obtain more information about these changes after rTMS, we measured changes in several CA enzymes, proteins, and trace metals in their blood plasma, erythrocytes, and saliva.

METHODS

Ninety-three patients with taste and smell dysfunction were studied before and after rTMS in an open clinical trial. Before and after rTMS, we measured erythrocyte CA I, II and salivary CA VI, zinc and copper in parotid saliva, blood plasma, and erythrocytes, and appearance of novel salivary proteins by using mass spectrometry.

RESULTS

After rTMS, CA I, II and CA VI activity and zinc and copper in saliva, plasma, and erythrocytes increased with significant sensory benefit. Novel salivary proteins were induced at an m/z value of 21.5K with a repetitive pattern at intervals of 5K m/z.

CONCLUSIONS

rTMS induced biochemical changes in specific enzymatic activities, trace metal concentrations, and induction of novel salivary proteins, with sensory improvement in patients with taste and smell dysfunction. Because patients with several neurologic disorders exhibit taste and smell dysfunction, including Parkinson disease, Alzheimer disease, and multiple sclerosis, and because rTMS improved their clinical symptoms, the biochemical changes we observed may be relevant not only in our patients with taste and smell dysfunction but also in patients with neurologic disorders with these sensory abnormalities.

摘要

简介

重复经颅磁刺激(rTMS)已被用于治疗多种疾病的症状；这种治疗会引起生化变化。rTMS 治疗味觉和嗅觉功能障碍患者的初步研究改善了感觉功能，并增加了唾液碳酸酐酶(CA) VI 和红细胞 CA I、II。为了获得 rTMS 后这些变化的更多信息，我们测量了血液血浆、红细胞和唾液中几种 CA 酶、蛋白质和痕量金属的变化。

方法

93 例味觉和嗅觉功能障碍患者在一项开放临床试验中接受 rTMS 治疗前后进行了研究。在 rTMS 治疗前后，我们测量了红细胞 CA I、II 和唾液 CA VI、锌和铜在腮腺唾液、血浆和红细胞中的含量，以及使用质谱法测量唾液中新型蛋白质的出现。

结果

rTMS 后，唾液、血浆和红细胞中的 CA I、II 和 CA VI 活性以及锌和铜增加，感觉功能明显改善。在 m/z 值为 21.5K 处诱导出具有重复模式的新型唾液蛋白，间隔为 5K m/z。

结论

rTMS 诱导了特定酶活性、痕量金属浓度和新型唾液蛋白的生化变化，味觉和嗅觉功能障碍患者的感觉改善。由于几种神经障碍疾病患者表现出味觉和嗅觉功能障碍，包括帕金森病、阿尔茨海默病和多发性硬化症，并且 rTMS 改善了他们的临床症状，因此我们观察到的生化变化不仅与我们的味觉和嗅觉功能障碍患者相关，而且与具有这些感觉异常的神经障碍患者相关。