嗅觉功能障碍患者鼻黏液中的环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）与嗅觉丧失严重程度的关系。

cAMP and cGMP in nasal mucus related to severity of smell loss in patients with smell dysfunction.

作者信息

Henkin R I, Velicu I

机构信息

Center for Molecular Nutritional and SensoryDisorders, The Taste and Smell Clinic, Washington, DC 20016, USA.

出版信息

Clin Invest Med. 2008;31(2):E78-84. doi: 10.25011/cim.v31i2.3367.

DOI:10.25011/cim.v31i2.3367

PMID:18377764

Abstract

PURPOSE

To evaluate nasal mucus levels of cAMP and cGMP in patients with taste and smell dysfunction with respect to severity of their smell loss.

METHODS

cAMP and cGMP were measured in nasal mucus using a sensitive spectrophotometric 96 plate ELISA technique. Smell loss was measured in patients with taste and smell dysfunction by standardized psychophysical measurements of olfactory function and classified by severity of loss into four types from most severe to least severe such that anosmia > Type I hyposmia > Type II hyposmia > Type III hyposmia. Measurements of nasal mucus cyclic nucleotides and smell loss were made independently.

RESULTS

As smell loss severity increased stepwise cAMP and cGMP levels decreased stepwise [cAMP, cGMP (in pmol/ml); anosmia - 0.004, 0.008: Type I hyposmia - 0.12+/-0.03, 0.10+/-0.03: Type II hyposmia - 0.15+/-0.02, 0.16+/-0.01: Type III hyposmia - 0.23+/-0.05, 0.20+/-0.15].

CONCLUSIONS

These results confirm the association of biochemical changes in cyclic nucleotides with systematic losses of smell acuity. These results confirm the usefulness of the psychophysical methods we defined to determine the systematic classification of smell loss severity. These changes can form the basis for the biochemical definition of smell loss among some patients with smell loss as well as for their therapy.

摘要

目的

评估味觉和嗅觉功能障碍患者鼻腔黏液中cAMP和cGMP水平与嗅觉丧失严重程度之间的关系。

方法

采用灵敏的分光光度96孔酶联免疫吸附测定（ELISA）技术测量鼻腔黏液中的cAMP和cGMP。通过对嗅觉功能进行标准化的心理物理学测量来评估味觉和嗅觉功能障碍患者的嗅觉丧失情况，并根据丧失的严重程度从最严重到最不严重分为四种类型，即嗅觉缺失>Ⅰ型嗅觉减退>Ⅱ型嗅觉减退>Ⅲ型嗅觉减退。鼻腔黏液环核苷酸的测量和嗅觉丧失的测量是独立进行的。

结果

随着嗅觉丧失严重程度逐步增加，cAMP和cGMP水平逐步降低[cAMP、cGMP（单位：pmol/ml）；嗅觉缺失-0.004，0.008；Ⅰ型嗅觉减退-0.12±0.03，0.10±0.03；Ⅱ型嗅觉减退-0.15±—0.02，0.16±0.01；Ⅲ型嗅觉减退-0.23±0.05，0.20±0.15]。