Desgranges C, Campan M, Gadeau A P, Guerineau N, Mollard P, Razaka G
Unité de Cardiologie INSERM U8, Pessac, France.
Biochem Pharmacol. 1991;41(6-7):1045-54. doi: 10.1016/0006-2952(91)90213-o.
8-(N,N-Diethylamino)octyl-3,4,5-trimethoxybenzoate (TMB-8), a putative inhibitor of intracellular calcium mobilization, causes a dose-dependent inhibition of serum-induced proliferation of arterial smooth muscle cells in culture. Neither early rise in cytosolic calcium concentration nor induction of early induced cell cycle dependent genes (c-fos, ornithine decarboxylase) are inhibited after serum stimulation in presence of 100 microM TMB-8. In contrast, expression of thymidine kinase, a gene normally induced in late-G1 phase, is entirely inhibited by TMB-8. Taken together with flow cytometry studies, these results indicate that TMB-8 blocks cell cycle progression in mid- or late-G1 phase by a mechanism not directly related to early responses to serum stimulation since TMB-8 is also effective when introduced several hours after serum stimulation.
8-（N，N-二乙氨基）辛基-3,4,5-三甲氧基苯甲酸酯（TMB-8）是一种推测的细胞内钙动员抑制剂，可对培养的动脉平滑肌细胞的血清诱导增殖产生剂量依赖性抑制作用。在100微摩尔TMB-8存在的情况下，血清刺激后，胞质钙浓度的早期升高和早期诱导的细胞周期依赖性基因（c-fos、鸟氨酸脱羧酶）的诱导均未受到抑制。相反，胸苷激酶（一种通常在G1期晚期诱导的基因）的表达完全被TMB-8抑制。结合流式细胞术研究，这些结果表明，TMB-8通过一种与血清刺激早期反应不直接相关的机制阻断G1期中期或晚期的细胞周期进程，因为在血清刺激数小时后引入TMB-8也有效。
