β-葡聚糖增强人结肠癌细胞 SNU-C4 的细胞凋亡。
beta-Glucan enhanced apoptosis in human colon cancer cells SNU-C4.
机构信息
Department of Food and Nutrition, Dongduk Women's University, 23-1 Wolgok-dong Sungbuk-gu, Seoul 136-714, Korea.
出版信息
Nutr Res Pract. 2009 Fall;3(3):180-4. doi: 10.4162/nrp.2009.3.3.180. Epub 2009 Sep 30.
The apoptotic effect of bacteria-derived beta-glucan was investigated in human colon cancer cells SNU-C4 using terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcription-polymerase chain reaction (RT-PCR) expressions of Bcl-2, Bax, and Caspase-3 genes, and assay of caspase-3 enzyme activity. beta-Glucan of 10, 50, and 100 microg/mL decreased cell viability in a dose-dependent manner with typical apoptotic characteristics, such as morphological changes of chromatin condensation and apoptotic body formation from TUNEL assay. In addition, beta-glucan (100 microg/mL) decreased the expression of Bcl-2 by 0.6 times, whereas the expression of Bax and Caspase-3 were increased by 3.1 and 2.3 times, respectively, compared to untreated control group. Furthermore, the caspase-3 activity in the beta-glucan-treated group was significantly increased compared to those in control group (P < 0.05). Bacterial derived beta-glucan could be used as an effective compound inducing apoptosis in human colon cancer.
采用末端脱氧核苷酸转移酶（TdT）介导的 dUTP 缺口末端标记（TUNEL）检测、逆转录-聚合酶链反应（RT-PCR）检测 Bcl-2、Bax 和 Caspase-3 基因表达以及 Caspase-3 酶活性检测的方法，研究了细菌来源的β-葡聚糖对人结肠癌细胞 SNU-C4 的凋亡作用。β-葡聚糖以 10、50 和 100 μg/ml 的剂量依赖性方式降低细胞活力，并具有典型的凋亡特征，如 TUNEL 检测到的染色质浓缩和凋亡小体形成的形态变化。此外，β-葡聚糖（100 μg/ml）使 Bcl-2 的表达降低了 0.6 倍，而 Bax 和 Caspase-3 的表达分别增加了 3.1 倍和 2.3 倍，与未处理的对照组相比。此外，与对照组相比，β-葡聚糖处理组的 Caspase-3 活性显著增加（P < 0.05）。细菌来源的β-葡聚糖可作为诱导人结肠癌细胞凋亡的有效化合物。
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