Budijanto Slamet, Nurtiana Winda, Muniroh Amirotul, Kurniati Yeni, Nuraida Lilis, Priosoeryanto Bambang Pontjo, Annisa Dewi Fitriya Nur, Ardiansyah Ardiansyah, Yuliana Nancy Dewi, Safrida Safrida, Shirakawa Hitoshi
Department of Food Science and Technology, IPB University, Dramaga, Bogor, 16680, Indonesia.
Department of Food Technology, University of Sultan Ageng Tirtayasa, Raya Jakarta Km 4 Pakupatan, Serang, 42124, Indonesia.
Heliyon. 2023 Jul 28;9(8):e18528. doi: 10.1016/j.heliyon.2023.e18528. eCollection 2023 Aug.
This research aims to identify the effects of the administration of a black rice bran diet on colorectal cancer in dextran sodium sulfate and azoxymethane-induced BALB/c mice. The research was conducted on three groups consisting of eight Balb/c mice: two groups were fed with carcinogens, and the third group, referred to as the normal group, was supplied with Isotonic NaCl 0.9% intraperitoneally. One group fed with carcinogens was supplied a standard AIN 1993 M diet modified with black rice bran as a substitute of fibre source, while the other two mice groups were fed the standard diet (AIN-93M) containing cellulose fibre. At the 17th week, all mice were euthanized; their colonic sections were taken for histopathological evaluation, and cecum for short-chain fatty acids concentration, total lactic acid bacteria, pH and β-glucuronidase activity evaluations. The results show an increase in the total lactic acid bacteria and short-chain fatty acids in the mice group fed with rice bran. Consequently, pH value and β-glucuronidase activity had decreased. Histopathological evaluation of mucosal tissue exhibited inhibition of the tumor growth rate in the mice groups fed rice bran compared to the group supplied with the standard diet. Furthermore, the proliferating cell nuclear antigen expression had decreased significantly, while expression of caspase-8 and caspase-3 had increased notably, in the group fed with a rice bran diet. These results suggest that black rice bran can effectively inhibit colon carcinogenesis. The potential of black rice bran as a source of fibre has not been studied in detail regarding the inhibition mechanism of colorectal cancer cells; further investigation in this field could provide valuable information about new strategies to prevent colorectal cancer. This strand of research is very important to developing preventive methods against cancer and promoting the concept of healthy products, including functional foods.
本研究旨在确定在葡聚糖硫酸钠和氧化偶氮甲烷诱导的BALB/c小鼠中，给予黑米糠饮食对结直肠癌的影响。该研究对三组共八只Balb/c小鼠进行：两组给予致癌物，第三组为正常组，腹腔注射0.9%的等渗氯化钠。一组给予致癌物的小鼠喂食用黑米糠替代纤维来源改良的标准AIN 1993 M饮食，而另外两组小鼠喂食含纤维素纤维的标准饮食（AIN-93M）。在第17周时，所有小鼠实施安乐死；取其结肠切片进行组织病理学评估，取盲肠进行短链脂肪酸浓度、总乳酸菌、pH值和β-葡萄糖醛酸酶活性评估。结果显示，喂食米糠的小鼠组中总乳酸菌和短链脂肪酸增加。因此，pH值和β-葡萄糖醛酸酶活性降低。与喂食标准饮食的组相比，喂食米糠的小鼠组黏膜组织的组织病理学评估显示肿瘤生长速率受到抑制。此外，喂食米糠饮食的组中增殖细胞核抗原表达显著降低，而半胱天冬酶-8和半胱天冬酶-3的表达显著增加。这些结果表明，黑米糠可有效抑制结肠癌发生。关于黑米糠作为纤维来源对结直肠癌细胞的抑制机制尚未进行详细研究；该领域的进一步研究可为预防结直肠癌的新策略提供有价值的信息。这一系列研究对于开发癌症预防方法以及推广包括功能性食品在内的健康产品概念非常重要。
