去甲肾上腺素可诱导培养的血浆胶质瘤细胞中胶质细胞特异性酶活性。
Norepinephrine induces glial-specific enzyme activity in cultured plasma glioma cells.
作者信息
McMorris F A
出版信息
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4501-4. doi: 10.1073/pnas.74.10.4501.
Abstract
Addition of 1 micronM 1-norepinephrine to cultures of C6TK- rat glioma cells caused a 2-fold increase in specific activity of the glial-specific enzyme 2':3'-cyclic nucleotide 3'-phosphohydrolase (nucleoside-2':3'-cyclic-phosphate 3'-nucleotidohydrolase, EC 3.1.4.16). Specific activity could also be stimulated by analogues of 3':5'-cyclic AMP, and the effect of norepinephrine could be blocked by beta-adrenergic receptor antagonists but not by alpha-adrenergic antagonists. Norepinephrine or cyclic AMP analogues also increased the specific activity of this enzyme in other clones of glioma and Schwannoma cells and in glioma X neuroblastoma cell hybrids. These results show that the stimulatory effect of norepinephrine on cyclic AMP concentrations in glioma cells leads ultimately to a stimulation of glial-specific cell funtion.
摘要
向C6TK -大鼠胶质瘤细胞培养物中添加1微摩尔的1 -去甲肾上腺素，可使神经胶质特异性酶2':3'-环核苷酸3'-磷酸水解酶（核苷-2':3'-环磷酸3'-核苷酸水解酶，EC 3.1.4.16）的比活性增加两倍。3':5'-环磷酸腺苷的类似物也能刺激比活性，去甲肾上腺素的作用可被β -肾上腺素能受体拮抗剂阻断，但不能被α -肾上腺素能拮抗剂阻断。去甲肾上腺素或环磷酸腺苷类似物还能增加该酶在其他胶质瘤和施万细胞瘤细胞克隆以及胶质瘤X神经母细胞瘤细胞杂交体中的比活性。这些结果表明，去甲肾上腺素对胶质瘤细胞中环磷酸腺苷浓度的刺激作用最终导致神经胶质特异性细胞功能的刺激。
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Norepinephrine induces glial-specific enzyme activity in cultured plasma glioma cells.去甲肾上腺素可诱导培养的血浆胶质瘤细胞中胶质细胞特异性酶活性。
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4501-4. doi: 10.1073/pnas.74.10.4501.
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