Davaki P, Lantos P L
Br J Exp Pathol. 1980 Dec;61(6):655-60.
A comparative study of the cellular composition and ultrastructural features of rat brain tumours produced by early and late passage of neoplastic glial cells was carried out. The cells injected intracerebrally were of a clone neoplastic astrocytes (A15A5) derived from a mixed glioma induced transplacentally by N-ethyl-N-nitrosourea (ENU) in a BD-IX rat. The neoplastic cells were maintained for various lengths of time in vitro and then injected (5 X 10(5) cells) into the left frontal lobe of the brains of syngeneic rats, resulting in a 100% tumour yield. Gliomas produced by cells of earlier passages were less malignant and contained better differentiated astrocytes than those produced by cells of later passages. There were also differences in size, shape, extracellular space, presence of haemorrhage and necrosis, vascularity and invasion of the surrounding tissues. Thus the morphological features of transplanted tumours in vivo correlated well with the in vitro behaviour of neoplastic glial cells.
对由肿瘤性神经胶质细胞早期传代和晚期传代产生的大鼠脑肿瘤的细胞组成和超微结构特征进行了比较研究。脑内注射的细胞是源自BD-IX大鼠经N-乙基-N-亚硝基脲（ENU）经胎盘诱导的混合胶质瘤的克隆性肿瘤性星形胶质细胞（A15A5）。将肿瘤细胞在体外维持不同时间，然后（5×10⁵个细胞）注射到同基因大鼠脑的左额叶，肿瘤发生率为100%。早期传代细胞产生的胶质瘤恶性程度较低，与晚期传代细胞产生的胶质瘤相比，含有分化更好的星形胶质细胞。在大小、形状、细胞外空间、出血和坏死的存在、血管形成以及对周围组织的侵袭方面也存在差异。因此，体内移植肿瘤的形态学特征与肿瘤性神经胶质细胞的体外行为密切相关。
