脂质双层覆盖与穿透二氧化硅胶体晶体。

Lipid bilayer blanketing versus penetrating silica colloidal crystals.

机构信息

Department of Chemistry & Biochemistry, University of Arizona, Tucson, Arizona 85721, USA.

出版信息

Langmuir. 2010 Feb 16;26(4):2196-9. doi: 10.1021/la9038914.

PMID:20092346

Abstract

Fluorescence intensities and diffusion coefficients were measured for a labeled lipid in 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) bilayers deposited by vesicle fusion on two types of silica colloidal crystals, one made from 145 nm particles and the other from 490 nm particles. The bilayer is shown to blanket the top of the silica colloidal crystal for the 145 nm case and to coat the entire surface area for the 450 nm case. The diffusion coefficient of the labeled lipid is shown to be the same in both cases, showing that the diffusion coefficient is not an indicator of bilayer penetration.

摘要

荧光强度和扩散系数分别对标记脂质在 1-棕榈酰-2-油酰基-sn-甘油-3-磷酸胆碱（POPC）双层膜中的进行了测量，该双层膜是由脂质体融合在两种类型的二氧化硅胶体晶体上形成的，其中一种胶体晶体由 145nm 的颗粒组成，另一种胶体晶体由 490nm 的颗粒组成。实验结果表明，在 145nm 的情况下，双层膜覆盖了整个二氧化硅胶体晶体的顶部，而在 450nm 的情况下，双层膜覆盖了整个胶体晶体的表面。此外，实验结果还表明，两种情况下标记脂质的扩散系数相同，这表明扩散系数不是双层膜穿透的指标。