Graduate Program in Bioengineering, Seoul National University, Seoul 151-744, Republic of Korea.
Biosens Bioelectron. 2010 Mar 15;25(7):1566-70. doi: 10.1016/j.bios.2009.10.013. Epub 2009 Nov 14.
To detect organophosphate chemicals, which are used both as pesticides and as nerve agents, a novel biosensor based on organophosphorus hydrolase was developed. By using mesoporous carbon (MC) and carbon black (CB) as an anodic layer, the sensitivity of the sensor to p-nitrophenol (PNP), which is the product of the organophosphorus hydrolase reaction, was greatly improved. The MC/CB/glass carbon (GC) layer exhibited an enhanced amperometric response relative to a carbon nanotube (CNT)-modified electrode because it promoted electron transfer of enzymatically generated phenolic compounds (p-nitrophenol). The well-ordered nanopores, many edge-plane-like defective sites (EDSs), and high surface area of the MC resulted in increased sensitivity, and allowed for nanomolar-range detection of the analyte paraoxon. Thus, MCs are suitable for use in real-time biosensors. Under the optimized experimental conditions, the biosensor had a detection limit of 0.12 microM (36 ppb) and a sensitivity of 198 nA/microM for paraoxon.
为了检测有机磷化学物质，这些化学物质既可用作农药，也可用作神经毒剂，研究人员开发了一种基于有机磷水解酶的新型生物传感器。通过使用介孔碳 (MC) 和炭黑 (CB) 作为阳极层，大大提高了传感器对有机磷水解酶反应产物对硝基苯酚 (PNP) 的灵敏度。与经过碳纳米管 (CNT) 修饰的电极相比，MC/CB/玻璃碳 (GC) 层表现出增强的电流响应，因为它促进了酶促生成的酚类化合物（对硝基苯酚）的电子转移。MC 的有序纳米孔、许多边缘平面状缺陷位 (EDS) 和高表面积导致了灵敏度的增加，并允许对分析物对氧磷进行纳摩尔范围的检测。因此，MC 适用于实时生物传感器。在优化的实验条件下，该生物传感器对氧磷的检测限为 0.12 microM（36 ppb），灵敏度为 198 nA/μM。
