Li Shanshan, Luo Qingying, Liu Yaowen, Zhang Zhiqing, Shen Guanghui, Wu Hejun, Chen Anjun, Liu Xingyan, Zhang Aidong
College of Food Science, Sichuan Agricultural University, Ya'an 625014, China.
Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, China.
Polymers (Basel). 2017 Aug 12;9(8):359. doi: 10.3390/polym9080359.
This paper presents the fabrication of a molecularly imprinted, polymer-based disposable electrochemical sensor for paraoxon (PO) determination. The sensor was based on a screen-printed carbon electrode (SPCE) modified with a surface molecularly imprinted poly (-aminothiophenol) (PATP)/gold nanoparticles (AuNPs) composite film, which consisted of a PATP outer layer and an AuNPs inner layer. We report a novel strategy, combining surface molecularly imprinting and self-assembly directed electro-polymerization with high densely imprinting PO molecules in the PATP/AuNPs film. Firstly, AuNPs were in situ electrodeposited at the electrode surface, and then assembled with electropolmerizable functional monomer -aminothiophenol (ATP). Subsequently, PO molecules were assembled onto the ATP monolayer-modified AuNPs, forming a basis of surface molecular imprinting. After that, replenished PO molecules were embedded in the PATP/AuNPs film by PO and the ATP molecular self-assembly directed electro-polymerization in the polymerization precursor mixture. The resulting imprinted PATP/AuNPs/SPCE possesses high sensitivity, affinity, and selectivity toward PO, with a low detection limit of 1 × 10 M. The proposed sensor was successfully applied for the determination of PO in fruit and vegetables, giving satisfactory recoveries. The strategy reported herein can be further expected to fabricate various molecular imprinted sensors for the determination of other pesticide residuals.
本文介绍了一种用于对氧磷（PO）测定的基于聚合物的分子印迹一次性电化学传感器的制备方法。该传感器基于丝网印刷碳电极（SPCE），其表面修饰有表面分子印迹聚（-氨基硫酚）（PATP）/金纳米颗粒（AuNPs）复合膜，该复合膜由PATP外层和AuNPs内层组成。我们报道了一种新颖的策略，即将表面分子印迹与自组装定向电聚合相结合，在PATP/AuNPs膜中高密集地印迹PO分子。首先，AuNPs在电极表面原位电沉积，然后与可电聚合的功能单体-氨基硫酚（ATP）组装。随后，PO分子组装到ATP单层修饰的AuNPs上，形成表面分子印迹的基础。之后，通过PO和ATP分子在聚合前体混合物中的自组装定向电聚合，将补充的PO分子嵌入PATP/AuNPs膜中。所得的印迹PATP/AuNPs/SPCE对PO具有高灵敏度、亲和力和选择性，检测限低至1×10 M。所提出的传感器成功应用于水果和蔬菜中PO的测定，回收率令人满意。本文报道的策略有望进一步用于制备各种用于测定其他农药残留的分子印迹传感器。
