Senbua Witsanu, Mearnchu Jhirat, Wichitwechkarn Jesdawan
Department of Biotechnology, Faculty of Engineering and Industrial Technology, Silpakorn University, Nakhon Pathom, 73000, Thailand.
Department of Electrical Engineering, Faculty of Engineering and Industrial Technology, Silpakorn University, Nakhon Pathom, 73000, Thailand.
Biotechnol Rep (Amst). 2020 Jun 23;27:e00495. doi: 10.1016/j.btre.2020.e00495. eCollection 2020 Sep.
Due to high contamination of organophosphate (OP) insecticides in agricultural products and the environment, efficient and convenient devices for their monitoring are necessary. Here, a simple, inexpensive, efficient, and easy-to-use absorbance-based biosensor was fabricated utilizing recombinant methyl parathion hydrolase fused with glutathione--transferase (MPH-GST), covalently immobilized onto a chitosan film-coated polystyrene microplate, for the detection of methyl parathion (MP) as a representative of OPs. Having been connected to the transducer system designed to work through an Arduino microcontroller, the biosensor could detect MP as efficiently as the conventional methods, with the detection limit of 0.1 μM, the lowest value ever reported for this method. It was stable at 25 °C for 30 days, could function 100 rounds repetitively, and yielded high recovery with real samples. Hence, this simply designed MPH-GST biosensor could be an easy and inexpensive alternative for efficient OP screening at site to help control its contamination.
由于农产品和环境中有机磷（OP）杀虫剂的高污染，需要高效便捷的监测设备。在此，利用与谷胱甘肽 - S - 转移酶（GST）融合的重组甲基对硫磷水解酶（MPH - GST），共价固定在壳聚糖膜包被的聚苯乙烯微孔板上，制备了一种简单、廉价、高效且易于使用的基于吸光度的生物传感器，用于检测作为有机磷代表的甲基对硫磷（MP）。该生物传感器连接到通过Arduino微控制器工作的换能器系统后，能够像传统方法一样高效地检测MP，检测限为0.1 μM，是该方法迄今报道的最低值。它在25°C下稳定30天，可重复运行100次，对实际样品具有高回收率。因此，这种设计简单的MPH - GST生物传感器可以成为现场高效有机磷筛查的一种简便且廉价的替代方法，有助于控制其污染。
