Goodrich Raymond P, Doane Suzann, Reddy Heather L
CaridianBCT Biotechnologies LLC, 1215 Quail Street, Lakewood, CO 80215, USA.
Biologicals. 2010 Jan;38(1):20-30. doi: 10.1016/j.biologicals.2009.10.016. Epub 2010 Jan 25.
The use of blood components has been a staple of transfusion medicine for several decades. Technologies for the processing and handling of blood, including separation of components from whole blood, are very well developed. Relative to blood safety, methods to detect the presence of pathogens and reduce the levels of donor white blood cells from whole blood are also well established in routine practice. The advantages which exist for the handling of whole blood by these methods have, for various reasons, not extended to the field of pathogen reduction technology (PRT). PRT methods have been developed and are now in routine use in various locations for addressing single donor or pooled plasma and platelet products. Several methods have also been in experimental development for the treatment of red blood cells as a separate component. The ability to treat whole blood in a fashion that would allow a single pathogen reduction and white blood cell inactivation step, to be followed by use of the product in the form of whole blood or separation into components, would afford several benefits from both a logistical and practical standpoint. This manuscript describes development efforts using a photochemical PRT method employing riboflavin and UV-Light (Mirasol PRT).
几十年来，血液成分的使用一直是输血医学的主要内容。血液处理和处理技术，包括从全血中分离成分，已经非常成熟。相对于血液安全，在常规实践中，检测病原体存在和降低全血中供体白细胞水平的方法也已确立。由于各种原因，通过这些方法处理全血所具有的优势并未扩展到病原体灭活技术（PRT）领域。PRT方法已经开发出来，目前在各地常规用于处理单采或混合血浆及血小板制品。也有几种方法正在进行实验开发，用于将红细胞作为单独成分进行处理。以一种允许进行单一病原体灭活和白细胞失活步骤，随后以全血形式使用该产品或将其分离成成分的方式处理全血，从后勤和实际角度来看将带来诸多益处。本手稿描述了使用采用核黄素和紫外线的光化学PRT方法（Mirasol PRT）的开发工作。
