塞浦路斯山羊中的朊病毒基因多态性及其与天然瘙痒病抗性或易感性的关系。

PrP gene polymorphisms in Cyprus goats and their association with resistance or susceptibility to natural scrapie.

机构信息

TSE Section, Laboratory of Animal Health, State Veterinary Laboratories, Veterinary Services, 1417 Nicosia, Cyprus.

出版信息

Vet J. 2011 Feb;187(2):245-50. doi: 10.1016/j.tvjl.2009.10.015. Epub 2010 Jan 25.

DOI:10.1016/j.tvjl.2009.10.015

PMID:20093056

Abstract

In contrast to scrapie in sheep, the genetic basis of susceptibility to scrapie in goats is not well understood. To study the association of prion protein (PrP) alleles with susceptibility to scrapie in goats in Cyprus, the coding sequence of the caprine PrP gene was determined in 717 goats, including 218 scrapie positive animals. Several novel polymorphisms were detected, such as a novel octarepeat variant and a stop codon mutation. Amino acids at codons 146 and 154 were associated with susceptibility to goat scrapie. Animals heterozygous for serine (S) and aspartate (D) at codon 146 were significantly under-represented in scrapie positive animals and no positive animals were found that were homozygous for these amino acids at codon 146. These results might provide the basis for genetic control of scrapie in Cypriot goats.

摘要

与绵羊的羊瘙痒病相反，山羊易感染瘙痒病的遗传基础还不是很清楚。为了研究在塞浦路斯山羊中朊病毒蛋白（PrP）等位基因与瘙痒病易感性的关系，在 717 只山羊中测定了山羊 PrP 基因的编码序列，包括 218 只瘙痒病阳性动物。检测到了一些新的多态性，例如新型的八重复变体和一个终止密码子突变。在 146 和 154 密码子处的氨基酸与山羊瘙痒病的易感性有关。在瘙痒病阳性动物中，146 密码子杂合丝氨酸（S）和天冬氨酸（D）的动物明显减少，在 146 密码子处这些氨基酸纯合的阳性动物未被发现。这些结果可能为塞浦路斯山羊瘙痒病的遗传控制提供基础。