Antibody Development, CMC Contrast, Copenhagen, Denmark.
Am J Clin Pathol. 2010 Feb;133(2):205-11. doi: 10.1309/AJCP12MHRTFZJPKW.
The overall purpose of the study was to demonstrate applicability of the DAKO dual-color chromogenic in situ hybridization (CISH) assay (DAKO Denmark, Glostrup) with respect to 4 fluorescence in situ hybridization (FISH) probes: MYC (c-MYC), EGFR, ERBB2 (HER2), and TOP2A. The study showed that the dual-color CISH assay can convert Texas red and fluorescein isothiocyanate (FITC) signals into chromogenic signals with an almost complete 1:1 conversion ratio. Agreement studies between the FISH assays for HER2 and TOP2A and the corresponding CISH conversion assays showed 100% concordance (kappa values of 1.0) between the CISH and FISH methods for HER2 and TOP2A status. The correlations of the gene copy number to centromere-17 ratios were similarly high, with a correlation coefficient (r) for HER2 and TOP2A of more than 0.95. Owing to the relatively small number of specimens in this study, it is important that the data are confirmed in a larger study.
本研究的总体目的是展示 DAKO 双色显色原位杂交（CISH）检测（丹麦 Glostrup 的 DAKO 公司）对于 4 种荧光原位杂交（FISH）探针：MYC（c-MYC）、EGFR、ERBB2（HER2）和 TOP2A 的适用性。研究表明，双色 CISH 检测可以将 Texas 红和异硫氰酸荧光素（FITC）信号转化为显色信号，转化率几乎为 1:1。针对 HER2 和 TOP2A 的 FISH 检测与相应的 CISH 转化检测之间的一致性研究表明，CISH 与 FISH 方法在 HER2 和 TOP2A 状态方面具有 100%的一致性（kappa 值为 1.0）。基因拷贝数与着丝粒 17 比值的相关性也很高，HER2 和 TOP2A 的相关系数（r）超过 0.95。由于本研究中标本数量相对较少，因此在更大的研究中确认这些数据非常重要。
