Bernstein Center for Computational Neuroscience, Humboldt University, 10115 Berlin, Germany.
Science. 2010 Jan 22;327(5964):474-7. doi: 10.1126/science.1182773.
In vivo intracellular recordings of hippocampal neurons reveal the occurrence of fast events of small amplitude called spikelets or fast prepotentials. Because intracellular recordings have been restricted to anesthetized or head-fixed animals, it is not known how spikelet activity contributes to hippocampal spatial representations. We addressed this question in CA1 pyramidal cells by using in vivo whole-cell recording in freely moving rats. We observed a high incidence of spikelets that occurred either in isolation or in bursts and could drive spiking as fast prepotentials of action potentials. Spikelets strongly contributed to spiking activity, driving approximately 30% of all action potentials. CA1 pyramidal cell firing and spikelet activity were comodulated as a function of the animal's location in the environment. We conclude that spikelets have a major impact on hippocampal activity during spatial exploration.
在体海马神经元的细胞内记录揭示了快速小振幅事件的发生，这些事件被称为棘波或快速前峰。由于细胞内记录仅限于麻醉或头部固定的动物，因此尚不清楚棘波活动如何有助于海马空间表示。我们通过在自由活动的大鼠中使用在体全细胞膜片钳记录来解决这个问题。我们观察到棘波的发生率很高，它们可以单独发生或爆发，并可以作为动作电位的快速前峰驱动尖峰。棘波强烈地促进了放电活动，驱动了大约 30%的所有动作电位。CA1 锥体神经元的放电和棘波活动与动物在环境中的位置呈共调制关系。我们的结论是，在空间探索过程中，棘波对海马活动有重大影响。
