二核苷焦磷酸是T4诱导的RNA连接酶的底物。
Dinucleoside pyrophosphate are substrates for T4-induced RNA ligase.
作者信息
England T E, Gumport R I, Uhlenbeck O C
出版信息
Proc Natl Acad Sci U S A. 1977 Nov;74(11):4839-42. doi: 10.1073/pnas.74.11.4839.
Abstract
RNA ligase isolated from bacteriophage T4-infected Escherichia coli will utilize a number of different compounds with the general structure Ado-5'PP-X as substrates in an ATP-independent reaction. The P-X portions of these molecules are transferred to the 3'-hydroxyl of an oligoribonucleotide to form a phosphodiester bond, and the Ado-5'P (AMP) portion is released. AMP, CMP, GMP, UMP, dTMP, NMN, alphaNMN, reduced NMN, FMN, Rib-5P, phosphopantetheine, and cyanoethylphosphate all have been added to Cyd-3H3C from their corresponding AMP adducts. Contrary to the relative lack of specificity of RNA ligase for the P-X -group added, the failure of NADP+, deamino-NAD+, epsilonNCD+, epsilon NAD and CoA to react indicates that the enzyme shows a high degree of selectivity for the AMP portion of the substrate. The diversity of chemical groups that can be efficiently added suggests that this reaction of RNA ligase will prove useful for the modification of the 3' ends of RNA molecules.
摘要
从噬菌体T4感染的大肠杆菌中分离出的RNA连接酶，在不依赖ATP的反应中，会利用许多具有Ado-5'PP-X一般结构的不同化合物作为底物。这些分子的P-X部分转移到寡核糖核苷酸的3'-羟基上形成磷酸二酯键，而Ado-5'P（AMP）部分被释放。AMP、CMP、GMP、UMP、dTMP、NMN、αNMN、还原型NMN、FMN、核糖-5-磷酸、磷酸泛酰巯基乙胺和氰乙基磷酸都已从它们相应的AMP加合物添加到Cyd-3H3C中。与RNA连接酶对所添加的P-X基团相对缺乏特异性相反，NADP +、脱氨基-NAD +、εNCD +、εNAD和辅酶A不发生反应，这表明该酶对底物的AMP部分表现出高度的选择性。可以有效添加的化学基团的多样性表明，RNA连接酶的这种反应将被证明对RNA分子3'末端的修饰有用。
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