Kupper Jacqueline, Rentsch Katharina, Mittelholzer Andreas, Artho Romana, Meyer Sven, Kupferschmidt Hugo, Naegeli Hanspeter
University of Zürich, Institute of Veterinary Pharmacology and Toxicology, Winterthurerstrasse 260, CH-8057 Zurich, Switzerland.
J Vet Diagn Invest. 2010 Jan;22(1):119-22. doi: 10.1177/104063871002200125.
A heifer developed severe signs of acute gastrointestinal irritation 48 hr after ingesting fresh leaves of Colchicum autumnale growing on a damp meadow. Confirmation of the suspected toxicosis was obtained by detecting colchicine in serum and urine using liquid chromatography coupled with tandem mass spectrometry using atmospheric pressure chemical ionization. Although the serum colchicine concentration had declined to an apparently nontoxic level of 2.4 ng/ml, a more prominent concentration (640 ng/ml) indicative of colchicine poisoning was detected in the urine. This finding is consistent with the known toxicokinetic properties of colchicine, whereby a large volume of distribution results in low circulating blood concentrations and prolonged urinary excretion.
一头小母牛在食用了生长在潮湿草地上的秋水仙鲜叶48小时后，出现了严重的急性胃肠刺激症状。通过使用大气压化学电离的液相色谱-串联质谱法检测血清和尿液中的秋水仙碱，证实了疑似中毒情况。尽管血清中秋水仙碱浓度已降至明显无毒的2.4纳克/毫升水平，但在尿液中检测到了更高的浓度（640纳克/毫升），这表明存在秋水仙碱中毒。这一发现与秋水仙碱已知的毒代动力学特性相符，即分布容积大导致循环血液浓度低且尿排泄时间延长。
