Peking University Eye Center, Beijing, People's Republic of China.
Retina. 2010 Jun;30(6):966-70. doi: 10.1097/IAE.0b013e3181c71ea6.
The purpose of this study was to investigate the dynamic course of experimental thrombosis and thrombolysis of the retinal veins.
Dynamic changes in the blood flow in the retinal veins were documented on a digital recorder through a microscope-mounted video camera and were analyzed on a monitor by video playback. Photochemical thrombus formation was induced by intravenous injection of 30% Rose Bengal followed by endoillumination of individual branch retinal veins of the eyes of 20 anesthetized pigmented rabbits. Subsequently, 10 rabbits were treated with an infusion in an ear vein of 3 mg/kg recombinant tissue plasminogen activator, whereas 10 control rabbits were administered with similar volumes of normal saline solution. Occlusion and recanalization were confirmed histologically and assessed by video microscopy.
At the site exposed to light, photochemical injury resulted in the formation of a white thrombus, stagnation of blood flow, and subsequent complete venous occlusion in 20 rabbits. In this study, of the 10 animals in the recombinant tissue plasminogen activator treatment group, 9 (90%) showed evident thrombolysis, whereas none of the control group animals showed evident thrombolysis. The video showed that the massive thrombus disrupted into nonuniform fragments, which were quickly washed away by the blood flow, and the circulation was reestablished with no recurrence of secondary obstruction.
In vivo data suggest that video microscopy may provide a visual approach for observing the dynamic changes occurring during experimental thrombus formation and lysis by the early administration of recombinant tissue plasminogen activator; this approach may assist in future investigation of thrombus research of ocular fundus.
本研究旨在探讨视网膜静脉实验性血栓形成和溶栓的动态过程。
通过显微镜安装的摄像机将视网膜静脉内的血流动态变化记录在数字记录仪上，并通过视频回放在监视器上进行分析。通过静脉内注射 30%Rose Bengal 诱导光化学血栓形成，然后对 20 只麻醉色素兔的单个分支视网膜静脉进行内照明。随后，10 只兔子通过耳静脉输注 3mg/kg 重组组织型纤溶酶原激活剂进行治疗，而 10 只对照兔子给予相同体积的生理盐水溶液。通过组织学和视频显微镜评估闭塞和再通。
在暴露于光的部位，光化学损伤导致 20 只兔子形成白色血栓、血流停滞，随后静脉完全闭塞。在这项研究中，在重组组织型纤溶酶原激活剂治疗组的 10 只动物中，有 9 只（90%）表现出明显的溶栓作用，而对照组动物均未表现出明显的溶栓作用。视频显示，巨大的血栓破裂成不均匀的碎片，很快被血流冲走，循环恢复，没有继发性阻塞的复发。
体内数据表明，视频显微镜可能通过早期给予重组组织型纤溶酶原激活剂，为观察实验性血栓形成和溶解过程中的动态变化提供一种直观的方法；这种方法可能有助于未来对眼部眼底血栓研究的探索。
