Kimura M, Ushijima I, Hiraki M, Kimura M, Ono Nobufumi
Medicinal-Informatics and Research Unit, Faculty of Pharmaceutical Sciences, Fukuoka University, Jonan-ku, Fukuoka, Japan.
Methods Find Exp Clin Pharmacol. 2009 Nov;31(9):585-9. doi: 10.1358/mf.2009.31.9.1435462.
Combinations of caffeine with L-arginine or with taurine can enhance the effect of caffeine, but the mechanisms remain elusive. This study was designed to test the hypothesis that stimulant effects of central nervous system nitric oxide (NO) may explain the beneficial effect of caffeine on combinations with amino acid, L-arginine or taurine. Caffeine increased the spontaneous locomotor activity dose-dependently (2-10 mg/kg) in mice. The locomotor activity induced by caffeine at a dose of 2 mg/kg was enhanced by combined administration of L-arginine at a dose of 600 mg/kg, or taurine at a dose of 400 mg/kg, respectively. For both combinations, enhancement was significantly inhibited by pretreatment with N-nitro-L-arginine methyl ester (L-NAME) at a dose of 40 mg/kg. These results suggest that the enhancement induced by combining caffeine with amino acid might be regulated at least in part by NO in the central nervous system.
咖啡因与L-精氨酸或牛磺酸联合使用可增强咖啡因的效果，但其机制仍不清楚。本研究旨在验证以下假设：中枢神经系统一氧化氮（NO）的兴奋作用可解释咖啡因与氨基酸L-精氨酸或牛磺酸联合使用时的有益效果。咖啡因能使小鼠的自发运动活性呈剂量依赖性增加（2-10毫克/千克）。分别联合给予600毫克/千克的L-精氨酸或400毫克/千克的牛磺酸，可增强2毫克/千克咖啡因诱导的运动活性。对于这两种联合使用情况，40毫克/千克的N-硝基-L-精氨酸甲酯（L-NAME）预处理均能显著抑制这种增强作用。这些结果表明，咖啡因与氨基酸联合使用所诱导的增强作用可能至少部分受中枢神经系统中NO的调节。
