Department of Biological Sciences, Asahikawa Medical College, 2-1 Midorigaoka-higashi, Asahikawa, 078-8510, Japan.
J Assist Reprod Genet. 2010 Jan;27(1):41-7. doi: 10.1007/s10815-009-9381-z. Epub 2010 Jan 22.
This study was performed to investigate whether removal of cholesterol from the plasma membrane and collapse of the acrosome can prevent structural chromosome aberrations of paternal origin in mouse zygotes produced by intracytoplasmic sperm injection (ICSI).
Mouse spermatozoa were treated with methyl-beta-cyclodextrin (M beta CD) to remove cholesterol from the plasma membrane and with calcium ionophore A23187 to collapse the acrosome. Chromosomes of zygotes derived from M beta CD- and ionophore-treated spermatozoa were analyzed at the first mitotic metaphase.
Both chemical agents effectively induced the acrosome reaction. Incidence of structural chromosome aberrations in ICSI zygotes derived from M beta CD-treated spermatozoa was similar to that in zygotes produced by in vitro fertilization (IVF) with the same spermatozoa, but significantly lower compared to ICSI zygotes derived from acrosome-intact spermatozoa. Chromosome aberration rates in ICSI zygotes derived from ionophore-treated spermatozoa were evidently high compared to IVF zygotes.
Induction of the acrosome reaction through cholesterol efflux by M beta CD can prevent chromosome aberrations of paternal origin, while use of ionophore to induce the acrosome reaction exerts detrimental effect on paternal chromosomes in ICSI zygotes.
本研究旨在探讨通过去除质膜中的胆固醇和顶体崩溃是否可以防止通过胞质内精子注射（ICSI）产生的小鼠受精卵中父源结构染色体畸变。
用甲基-β-环糊精（MβCD）处理精子以去除质膜中的胆固醇，并使用钙离子载体 A23187 使顶体崩溃。用 MβCD 和离子载体处理的精子产生的受精卵在第一次有丝分裂中期分析染色体。
两种化学试剂均能有效诱导顶体反应。与来自完整顶体的精子的 ICSI 受精卵相比，来自 MβCD 处理的精子的 ICSI 受精卵的结构染色体畸变发生率相似，但明显低于来自体外受精（IVF）的受精卵。与 IVF 受精卵相比，来自离子载体处理的精子的 ICSI 受精卵的染色体畸变率明显较高。
通过 MβCD 去除胆固醇诱导顶体反应可以防止父源染色体畸变，而使用离子载体诱导顶体反应对 ICSI 受精卵中的父源染色体有不良影响。
