Alvarez R, Tusell L, Genescà A, Miró R, Benet J, Caballín M R, Egozcue J
Departament de Biologia Cel-lular i Fisiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Mol Hum Reprod. 1996 Jul;2(7):481-4. doi: 10.1093/molehr/2.7.481.
Incorporation of A23187 ionophore into the human-hamster fertilization system clearly improves the ability of human spermatozoa to penetrate zona-free hamster oocytes. Thus, an increasing number of laboratories working in human sperm cytogenetics have substituted classical incubation with Biggers-Whitten-Whittingham (BWW) medium plus human serum albumin (HSA) by pretreatment of spermatozoa with calcium ionophore A23187 which directly induces the acrosome reaction in spermatozoa. However, there have been no formal studies on the effects of this ionophore pretreatment. To determine whether calcium ionophore could affect the cytogenetic characteristics of human spermatozoa we compared A23187-treated spermatozoa with controls (only incubated with BWW + HSA) by analysing a total of 447 sperm chromosome complements from two normal donors. Our results show that there are no statistical differences in the frequency and the types of human sperm chromosomal abnormalities between the two methods of sperm treatment. Thus, ionophore A23187 seems not to affect the cytogenetic characteristics of human spermatozoa, and the results of laboratories using either sperm capacitation in BWW + HSA or acrosome reaction by calcium ionophore can be compared.
将A23187离子载体加入人-仓鼠受精系统中，明显提高了人类精子穿透去透明带仓鼠卵母细胞的能力。因此，越来越多从事人类精子细胞遗传学研究的实验室，用钙离子载体A23187对精子进行预处理，直接诱导精子发生顶体反应，取代了用比格斯-惠滕-惠廷厄姆（BWW）培养基加人血清白蛋白（HSA）进行的传统孵育。然而，尚未对这种离子载体预处理的效果进行正式研究。为了确定钙离子载体是否会影响人类精子的细胞遗传学特征，我们通过分析来自两名正常供体的总共447个精子染色体组型，将经A23187处理的精子与对照组（仅在BWW + HSA中孵育）进行了比较。我们的结果表明，两种精子处理方法在人类精子染色体异常的频率和类型上没有统计学差异。因此，离子载体A23187似乎不会影响人类精子的细胞遗传学特征，使用BWW + HSA中精子获能或钙离子载体诱导顶体反应的实验室结果可以进行比较。
