Podosinovikova N P, Beliaev V A, Dolgo-Saburov V B
Eksp Klin Farmakol. 2009 Nov-Dec;72(6):49-51.
A functional test using Daphnia magna Straus hydrobionts is proposed for studying the role of intermediatory relationships in the heart rate (HR) regulation. It is established that the M-cholinomimetic carbamylcholine increases for two hours and decreases after 24 hours the HR in D. magna. Caffeine (a nonselective antagonist of adenosine receptors) potentiates the action of carbamylcholine during the first hour and then ceases to influence the drug effect. Caffeine normalizes the HR rate D magna, which was decreased by the cholinolytic atropine and the beta-adrenolytic atenolol. The possibilities of using the proposed test for the investigation of intermediatory relationships in the HR regulation, rapid analysis of the cardiothropic action of xenobiotics, and the primary screening of drugs for the pharmacological correction of HR disturbances are discussed.
提出了一种使用大型溞水生生物进行的功能测试，以研究中介关系在心率（HR）调节中的作用。已确定M-胆碱能拟似物氨甲酰胆碱可使大型溞的心率在两小时内升高，并在24小时后降低。咖啡因（腺苷受体的非选择性拮抗剂）在第一小时增强氨甲酰胆碱的作用，然后不再影响药物效果。咖啡因可使被胆碱解磷定阿托品和β-肾上腺素能阻滞剂阿替洛尔降低的大型溞心率恢复正常。讨论了使用所提出的测试来研究心率调节中的中介关系、快速分析异生物素的心脏作用以及对心率紊乱进行药理纠正的药物初步筛选的可能性。
