Balthazart J, Foidart A, Surlemont C, Harada N
Laboratory of General and Comparative Biochemistry, University of Liège, Belgium.
Cell Tissue Res. 1991 Jan;263(1):71-9. doi: 10.1007/BF00318401.
The distribution of aromatase-immunoreactive cells was studied by immunocytochemistry in the mouse forebrain using a purified polyclonal antibody raised against human placental aromatase. Labeled perikarya were found in the dorso-lateral parts of the medial and tuberal hypothalamus. Positive cells filled an area extending between the subincertal nucleus in the dorsal part, the ventromedial hypothalamic nucleus in the ventral part, and the internal capsule and the magnocellular nucleus of the lateral hypothalamus in the lateral part. The same distribution was seen in the two strains of mice that were studied (Jackson and Swiss), and the number of immunoreactive perikarya did not seem to be affected by castration or testosterone treatment. No immunoreactivity could be detected in the medial regions of the preoptic area and hypothalamus; these were expected to contain the enzyme based on assays of aromatase activity performed in rats and on indirect autoradiographic evidence in mice. Our data raise questions concerning the distribution of aromatase in the brain and the mode of action of the centrally produced estrogens.
使用针对人胎盘芳香化酶产生的纯化多克隆抗体，通过免疫细胞化学方法研究了小鼠前脑中芳香化酶免疫反应性细胞的分布。在内侧和结节性下丘脑的背外侧部分发现了标记的神经元胞体。阳性细胞填充的区域在背侧的结节下核、腹侧的腹内侧下丘脑核以及外侧的内囊和下丘脑外侧的大细胞核之间延伸。在研究的两种小鼠品系（杰克逊和瑞士）中观察到相同的分布，免疫反应性神经元胞体的数量似乎不受去势或睾酮处理的影响。在视前区和下丘脑的内侧区域未检测到免疫反应性；基于在大鼠中进行的芳香化酶活性测定以及小鼠中的间接放射自显影证据，预计这些区域含有该酶。我们的数据引发了关于芳香化酶在大脑中的分布以及中枢产生的雌激素的作用方式的问题。
