Sheridan P J, Melgosa R T
Brain Res. 1983 Aug 29;273(2):285-9. doi: 10.1016/0006-8993(83)90852-1.
The nuclear uptake and retention of [3H]testosterone or one of its metabolites and the aromatization of testosterone to estrogen were examined in the Swiss--Webster mouse. Castrated male mice were injected with 0.2 micrograms of either [1 alpha, 2 alpha-3H(N)]testosterone or [1 beta, 2 beta-3H(N)]testosterone per 100 g of body weight and killed one and one-half hours later. The brains were removed and processed for autoradiography. A nuclear localization of testosterone or one of its metabolites was found in the nucleus (n) interstitialis striae terminalis, n. preopticus medialis, n. premamillaris ventralis and n. amygdaloideus medialis in animals injected with [1 alpha, 2 alpha-3H(N)]testosterone. In animals injected with [1 beta, 2 beta-3H(N)]testosterone a nuclear localization was found in only n. interstitialis striae terminalis, n. premamillaris ventralis and n. amygdaloideus medialis. The results suggest testosterone is aromatized to estrogen in n. preopticus medialis ventralis in the Swiss--Webster mouse. Together with previous data, these data suggest (1) the uptake and retention of testosterone or one of its androgenic metabolites and the aromatization of testosterone to estrogen varies between strains of mice and (2) there are two separate uptake and retention systems (receptors?) for testosterone and dihydrotestosterone in the brain in all animals studied thus far with autoradiographic techniques.
在瑞士-韦伯斯特小鼠中检测了[3H]睾酮或其代谢产物之一的核摄取和保留情况，以及睾酮向雌激素的芳香化作用。对去势雄性小鼠每100克体重注射0.2微克[1α,2α-3H(N)]睾酮或[1β,2β-3H(N)]睾酮，1个半小时后处死。取出大脑并进行放射自显影处理。在注射[1α,2α-3H(N)]睾酮的动物中，在终纹床核间质核、内侧视前核、腹侧乳头前核和内侧杏仁核中发现了睾酮或其代谢产物之一的核定位。在注射[1β,2β-3H(N)]睾酮的动物中，仅在终纹床核间质核、腹侧乳头前核和内侧杏仁核中发现了核定位。结果表明，在瑞士-韦伯斯特小鼠中，睾酮在内侧视前核腹侧被芳香化为雌激素。与先前的数据一起，这些数据表明：(1)睾酮或其雄激素代谢产物之一的摄取和保留以及睾酮向雌激素的芳香化在不同品系小鼠之间存在差异；(2)在迄今为止用放射自显影技术研究的所有动物的大脑中，存在两种独立的睾酮和双氢睾酮摄取和保留系统(受体？)。
