Anal Chem. 2010 Feb 15;82(4):1186-90. doi: 10.1021/ac902724y.
Thermally addressed immunoassay is developed to detect multiple proteins using phase change nanoparticles as thermal barcodes. The solid to liquid phase changes of nanoparticles absorb heat energy and generate sharp melting peaks, which are used as thermal signatures to determine the existence and concentration of proteins. Multiple proteins can be detected by using different types of nanoparticles in order to create a one-to-one correspondence between one type of nanoparticle and one type of protein. The fusion enthalpy that is proportional to the amount of phase change materials has been used to derive the amount of protein. The melting temperatures of nanoparticles are designed to be higher than 100 degrees C to avoid interference from species contained in the fluid. Thus, the use of thermal nanoparticles allows the detection of multiple low concentration proteins in a complex fluid such as cell lysate regardless of the color, salt concentration, and conductivity of the sample.
基于相变纳米粒子的热寻址免疫分析用于检测多种蛋白质。纳米粒子的固-液相变吸收热能并产生尖锐的熔融峰，可作为热特征来确定蛋白质的存在和浓度。通过使用不同类型的纳米粒子可以检测多种蛋白质，以便在一种纳米粒子和一种蛋白质之间建立一一对应的关系。与相变材料的量成正比的融合焓被用来推导蛋白质的量。纳米粒子的熔融温度设计得高于 100°C，以避免流体中所含物质的干扰。因此，热纳米粒子的使用可以在复杂的流体（如细胞裂解物）中检测多种低浓度蛋白质，而不受样品颜色、盐浓度和电导率的影响。
