NanoScience Technology Center, University of Central Florida, Orlando, Florida 32826, United States.
Anal Chem. 2011 Mar 15;83(6):2215-9. doi: 10.1021/ac103102h. Epub 2011 Feb 21.
A big challenge for multiplexed detection of cancer biomarkers is that biomarker concentrations in body fluid differs several orders of magnitude. Existing techniques are not suitable to detect low- and high-concentration biomarkers (protein and DNA) at the same time, and liquid chromatography or electrophoresis is used to separate or purify target biomarkers before analysis. This paper describes a new broad-range biomarker assay using solid to liquid phase change nanoparticles, where a panel of metallic nanoparticles (i.e., metals and eutectic alloys) are modified with a panel of ligands to establish a one-to-one correspondence and attached onto ligand-modified substrates by forming sandwiched complexes. The melting peak and fusion enthalpy of phase change nanoparticles during thermal analysis reflect the type and concentration of biomarkers, respectively. The thermal readout condition can be adjusted in such a way that multiple biomarkers with concentration difference over 3 orders of magnitude have been simultaneously detected under the same condition.
多指标癌症生物标志物检测面临的一个重大挑战是体液中的生物标志物浓度相差几个数量级。现有的技术不适于同时检测低浓度和高浓度的生物标志物（蛋白质和 DNA），通常在分析前使用液相色谱或电泳来分离或纯化目标生物标志物。本文介绍了一种新的广谱生物标志物检测方法，使用固-液相变纳米粒子，其中一组金属纳米粒子（即金属和共晶合金）用一组配体进行修饰，建立一一对应关系，并通过形成夹心复合物附着在配体修饰的基底上。热分析中相变纳米粒子的熔融峰和熔融焓分别反映了生物标志物的类型和浓度。可以调整热读取条件，使得在相同条件下能够同时检测浓度相差 3 个数量级以上的多种生物标志物。
