Zucker P F, Giles A, Chaulk E J, Archer M C
Department of Medical Biophysics, University of Toronto, Ontario Cancer Institute, Canada.
Carcinogenesis. 1991 Mar;12(3):405-8. doi: 10.1093/carcin/12.3.405.
The rat esophageal carcinogen N-nitrosomethylbenzylamine was shown to be highly toxic to rat esophageal epithelial (REE) cells in short-term primary culture. A significant level of cell killing could be observed at 10(-6) M. Several other esophageal carcinogens were also cytotoxic in a dose-dependent manner. Nitrosamines that do not produce esophageal tumors in the rat were generally unable to kill the esophageal cells. The results demonstrate that REE cells retain their metabolic capacity to activate specific nitrosamines to toxic metabolites. The culture system will be useful for mechanistic studies on the tissue specificity of these carcinogens, as well as to search for environmental agents that kill esophageal cells that may be involved in the causation of esophageal cancer.
大鼠食管癌致癌物N-亚硝基甲基苄胺在短期原代培养中对大鼠食管上皮（REE）细胞显示出高毒性。在10^(-6) M时可观察到显著的细胞杀伤水平。其他几种食管癌致癌物也呈剂量依赖性细胞毒性。在大鼠中不产生食管肿瘤的亚硝胺通常无法杀死食管细胞。结果表明，REE细胞保留了将特定亚硝胺激活为有毒代谢物的代谢能力。该培养系统将有助于对这些致癌物的组织特异性进行机制研究，以及寻找可杀死可能参与食管癌病因的食管细胞的环境因子。
