南美蕊木乳汁对亚马逊利什曼原虫的杀利什曼活性。
Leishmanicidal activity of Himatanthus sucuuba latex against Leishmania amazonensis.
作者信息
Soares Deivid C, Andrade Alexandre L S, Delorenzi Jan C, Silva Jefferson R A, Freire-de-Lima Leonardo, Falcão Camila A B, Pinto Angelo C, Rossi-Bergmann Bartira, Saraiva Elvira M
机构信息
Departamento de Imunologia, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
出版信息
Parasitol Int. 2010 Jun;59(2):173-7. doi: 10.1016/j.parint.2010.01.002. Epub 2010 Jan 20.
Himatanthus sucuuba (HsL) latex exhibited a potent leishmanicidal activity against intracellular amastigotes of Leishmania amazonensis, a causative agent of cutaneous leishmaniasis. HsL inhibited intracellular amastigote growth in a dose-dependent manner (IC(50)=15.7microg/mL). Moreover, HsL increased nitric oxide (NO) and Tumor Nuclear Factor-alpha (TNF-alpha) and decreased Transforming Growth Factor-beta (TGF-beta) production in macrophages. As assessed by plasma membrane integrity and mitochondrial activity, HsL showed low toxicity for host macrophages. HsL in vivo was active by the oral route, reducing the parasite load in established footpad lesions after only five doses. In summary, these findings support HsL as an interesting candidate for further evaluations regarding its potential application as a therapeutical agent against Leishmania.
南美蕊木（HsL）乳胶对皮肤利什曼病的病原体亚马逊利什曼原虫的细胞内无鞭毛体表现出强大的杀利什曼活性。HsL以剂量依赖性方式抑制细胞内无鞭毛体的生长（IC(50)=15.7微克/毫升）。此外，HsL可增加巨噬细胞中一氧化氮（NO）和肿瘤坏死因子-α（TNF-α）的产生，并减少转化生长因子-β（TGF-β）的产生。通过质膜完整性和线粒体活性评估，HsL对宿主巨噬细胞显示出低毒性。HsL在体内经口服具有活性，仅在五次给药后就能降低既定足垫病变中的寄生虫负荷。总之，这些发现支持将HsL作为一个有趣的候选物，进一步评估其作为抗利什曼治疗剂的潜在应用。
Zotero 插件