Chan C C, Roberge F G, Ni M, Zhang W, Nussenblatt R B
Laboratory of Immunology and Pathology, National Eye Institute, Bethesda, Maryland 20892.
Clin Immunol Immunopathol. 1991 May;59(2):201-7. doi: 10.1016/0090-1229(91)90018-6.
Müller cells have been shown to have a dual effect in vitro on autoimmune T helper lymphocytes. In a coculture system, Müller cells have a primary inhibitory effect on the proliferation of T lymphocytes. In conditions where their inhibitory action is suppressed, Müller cells can, however, stimulate T cells. In the present study we evaluated the in vivo effect of Müller cells on actively induced experimental autoimmune uveoretinitis (EAU). Ten millimoles of L-alpha-aminoadipic acid (L-AAA), a specific gliotoxic agent, was injected into the vitreous of one eye of Wistar-Furth (WF) rats (a low EAU responder) on the day of immunization. Control rats were injected similarly with phosphate-buffered saline alone. The rats were immunized with S-antigen in CFA or in CFA alone. The results demonstrate that the incidence of EAU increases twofold in the eyes receiving an intravitreal injection of L-AAA in comparison to the contralateral eyes not receiving an injection. No such difference in EAU incidence was observed in control animals. Some rats that had been immunized with CFA alone after an intravitreal injection of L-AAA demonstrated a small amount of retinal perivascular inflammatory cell infiltrate but did not develop typical EAU lesions. The retinal vasculature was normal on examination by fluorescein angiography after injection of L-AAA. These data suggest that Müller cells can influence the course of uveoretinitis through their interaction with T cells.
缪勒细胞在体外已被证明对自身免疫性辅助性T淋巴细胞有双重作用。在共培养系统中，缪勒细胞对T淋巴细胞的增殖具有主要抑制作用。然而，在其抑制作用被抑制的情况下，缪勒细胞可以刺激T细胞。在本研究中，我们评估了缪勒细胞对主动诱导的实验性自身免疫性葡萄膜视网膜炎（EAU）的体内作用。在免疫当天，将10毫摩尔的L-α-氨基己二酸（L-AAA，一种特异性神经毒性剂）注射到Wistar-Furth（WF）大鼠（EAU低反应者）一只眼睛的玻璃体内。对照大鼠同样仅注射磷酸盐缓冲盐水。大鼠用CFA中的S抗原或仅用CFA免疫。结果表明，与未接受注射的对侧眼睛相比，接受玻璃体内注射L-AAA的眼睛中EAU的发病率增加了两倍。在对照动物中未观察到EAU发病率的这种差异。一些在玻璃体内注射L-AAA后仅用CFA免疫的大鼠表现出少量视网膜血管周围炎性细胞浸润，但未发展出典型的EAU病变。注射L-AAA后通过荧光素血管造影检查发现视网膜血管系统正常。这些数据表明，缪勒细胞可通过与T细胞相互作用影响葡萄膜视网膜炎的病程。
