Department of Biochemistry, Faculty of Biology and Environmental Protection, University of Silesia, Katowice, Poland.
Chemosphere. 2010 Feb;78(9):1121-8. doi: 10.1016/j.chemosphere.2009.12.035. Epub 2010 Jan 21.
Automatic biodetector of water toxicity is a biosensor based on monitoring of catalytic activity of the nitrifying bacteria. To create a standardized biosensing system, development of the biofilm must be characterized to determine the prerequisites for its biological (biocatalytic) stability. In this paper, growth of biofilm comprising ammonium-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in the open cellular polyurethane material polyurethane sponge bioreactor has been investigated. Dynamics of the biofilm formation was estimated using AOB and NOB metabolic activity and the volume occupied by these two types of bacteria in the biofilm. Spectrophotometry liquid ion chromatography and image cytometry were used, respectively, for these measurements. A mathematical model of the dynamics of biofilm formation was established. These data indicate that open cellular polyurethane material is a good basis for the immobilization of nitrifying bacteria. Moreover, growth of the biofilm leads to its stable structural form, whose biocatalytic activity (12.29 for AOB and 6.84 micromol min(-1) for NOB) is constant in the long term.
水质毒性自动生物探测器是一种基于监测硝化细菌催化活性的生物传感器。为了创建标准化的生物传感系统，必须对生物膜的发展进行特征描述，以确定其生物（生物催化）稳定性的前提条件。在本文中，研究了在开放式多孔聚氨酯材料聚氨酯海绵生物反应器中包含氨氧化细菌（AOB）和亚硝酸盐氧化细菌（NOB）的生物膜的生长情况。使用 AOB 和 NOB 的代谢活性以及这两种细菌在生物膜中占据的体积来估计生物膜形成的动力学。分别使用分光光度法液相离子色谱法和图像细胞计数法进行了这些测量。建立了生物膜形成动力学的数学模型。这些数据表明，开放式多孔聚氨酯材料是固定硝化细菌的良好基础。此外，生物膜的生长导致其稳定的结构形式，其生物催化活性（AOB 为 12.29，NOB 为 6.84 umol min(-1)）在长期内保持不变。
