Agnieszka Nowak, Magdalena Pacwa-Płociniczak, Ariel Marchlewicz
Institute of Biology, Biotechnology and Environmental Protection, Faculty of Environmental Science, University of Silesia in Katowice, Jagiellońska St. 28, 40-032, Katowice, Poland.
Sci Rep. 2025 May 20;15(1):17441. doi: 10.1038/s41598-025-02321-3.
Contrast agents are very persistent compounds that are detected in increasing concentrations in sewage and water. This study aimed to determine whether the presence of iodinated contrast agent - diatrizoate impacts microorganisms in activated sludge. During the 100-day experiment, an attempt was made to find the most sensitive microbial indicator that would inform about changes in the structure or activity of microbial communities as a result of exposure to diatrizoate. Biochemical and genetic factors were tested, and it was found that the most sensitive indicators were dehydrogenase activity and indexes related to the oxidation of specific substrates. On the last day of the experiment, the values of all analysed functional diversity indicators were worse when a higher concentration of diatrizoate was added. Dehydrogenase activity was half lower, the Gini coefficient was 0.15 higher and the Shannon and Richness indices were 0.31 and 6 lower, respectively, compared to the control. Simultaneously, an increased intensity of glycogen, α-cyclodextrin, α-ketobutyric and D-malic acid oxidation was revealed. In conclusion, the long-term exposition to diatrizoate, affects microbial metabolism and causes significant adverse changes in their functional diversity. This work demonstrates the importance of long-term studies of the impact of pharmaceuticals on microorganisms in aquatic ecosystems.
造影剂是非常持久的化合物，在污水和水中的浓度不断增加。本研究旨在确定碘化造影剂泛影酸盐的存在是否会影响活性污泥中的微生物。在为期100天的实验中，试图找到最敏感的微生物指标，以了解由于接触泛影酸盐而导致的微生物群落结构或活性的变化。对生化和遗传因素进行了测试，发现最敏感的指标是脱氢酶活性和与特定底物氧化相关的指标。在实验的最后一天，添加较高浓度泛影酸盐时，所有分析的功能多样性指标的值都更差。与对照组相比，脱氢酶活性降低了一半，基尼系数高0.15，香农指数和丰富度指数分别低0.31和6。同时，糖原、α-环糊精、α-酮丁酸和D-苹果酸的氧化强度增加。总之，长期接触泛影酸盐会影响微生物代谢，并导致其功能多样性发生显著不利变化。这项工作证明了长期研究药物对水生生态系统中微生物影响的重要性。
