离子液体预处理生物质的糖化与商业酶混合物。
Saccharification of ionic liquid pretreated biomass with commercial enzyme mixtures.
机构信息
Department of Chemical Engineering, University of Toledo, 2801 W. Bancroft, Toledo, OH 43606, USA.
出版信息
Bioresour Technol. 2010 May;101(10):3561-6. doi: 10.1016/j.biortech.2009.12.066. Epub 2010 Jan 21.
The performance of Spezyme CP, a commercial cellulase system, and Primafast Luna CL, a textile bio-finishing enzyme, on hydrolysis of substrates following ionic liquid pretreatment was evaluated. Ionic liquid pretreatment of lignocellulosic biomass produces amorphous cellulose with little residual crystallinity and enhances its saccharification. The high crystallinity of native cellulose poses an impediment to enzyme hydrolysis of the cellulosic portion of biomass. Target substrates included poplar, switchgrass and Avicel, a highly crystalline cellulose substrate. Spezyme was found to hydrolyze glucan and xylan components completely in 24h with modest enzyme loadings. Primafast was found to principally hydrolyze glucan components of biomass. Xylanases added to the Primafast enzyme mixture appear to act synergistically to improve glucan hydrolysis with the poplar substrate.
评价了 Spezyme CP（一种商业纤维素酶系统）和 Primafast Luna CL（一种纺织生物整理酶）在离子液体预处理后水解底物的性能。离子液体预处理木质纤维素生物质会产生无定形纤维素，残余结晶度很小，从而提高了其糖化能力。天然纤维素的高结晶度对生物质中纤维素部分的酶水解构成了障碍。目标底物包括杨木、柳枝稷和 Avicel（一种高结晶度纤维素底物）。结果表明，Spezyme 在适度的酶用量下 24 小时内可完全水解葡聚糖和木聚糖成分。发现 Primafast 主要水解生物质中的葡聚糖成分。添加到 Primafast 酶混合物中的木聚糖酶似乎协同作用，提高了杨木底物的葡聚糖水解效率。
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