Milne Stephen B, Tallman Keri A, Serwa Remigiusz, Rouzer Carol A, Armstrong Michelle D, Marnett Lawrence J, Lukehart Charles M, Porter Ned A, Brown H Alex
[1] Department of Pharmacology, Vanderbilt University, Nashville, Tennessee, USA. [2] These authors contributed equally to this work.
Nat Chem Biol. 2010 Mar;6(3):205-207. doi: 10.1038/nchembio.311. Epub 2010 Jan 24.
Alkyne-modified phospholipids can be unambiguously identified and differentiated from native species in complex mixtures by formation of dicobalthexacarbonyl complexes. This reaction is specific for alkynes and is unaffected by other glycerophospholipid-related moieties. Enrichment of cells with alkyne-derivatized fatty acids or glycerophospholipids followed by solid-phase sequestration and release is a promising new method for unequivocally monitoring individual glycerophospholipids following incorporation into cells. This technique also facilitates lipidomic analysis of substrates and products.
通过形成二钴六羰基配合物，炔烃修饰的磷脂可以在复杂混合物中与天然物种进行明确鉴定和区分。该反应对炔烃具有特异性，不受其他甘油磷脂相关部分的影响。用炔烃衍生化的脂肪酸或甘油磷脂富集细胞，然后进行固相螯合和释放，是一种在将单个甘油磷脂掺入细胞后明确监测它们的有前途的新方法。该技术还便于对底物和产物进行脂质组学分析。
