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来自酿酒酵母的一种单链DNA结合蛋白能特异性识别自主复制序列（ARS）元件核心序列中富含胸腺嘧啶（T）的链，并区分突变序列。

A single-stranded DNA binding protein from S. cerevisiae specifically recognizes the T-rich strand of the core sequence of ARS elements and discriminates against mutant sequences.

作者信息

Schmidt A M, Herterich S U, Krauss G

机构信息

Laboratorium für Biochemie, Universität Bayreuth, Germany.

出版信息

EMBO J. 1991 Apr;10(4):981-5. doi: 10.1002/j.1460-2075.1991.tb08032.x.

DOI:10.1002/j.1460-2075.1991.tb08032.x
PMID:2009863
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC452742/
Abstract

A protein named ssARS-T binding protein has been purified from yeast that specifically binds to the T-rich strand of the consensus core sequence of yeast autonomously replicating sequence (ARS) elements. As assayed from gel mobility shift experiments the ssARS-T protein shows characteristics of a sequence specific single-stranded DNA binding protein. The complementary A-rich strand of the ARS core sequence is bound much more weakly and no binding can be detected for the double-stranded form of the core sequence. Three single base substitutions in the core sequence that are known to abolish ARS function in vivo also lead to weaker binding of the core sequence to the ssARS-T protein in vitro. The strong correlation between the binding of mutated sequences in vitro and the ARS properties of these sequences in vivo points to an essential function of the ssARS-T protein during replication initiation in yeast ARS elements.

摘要

一种名为ssARS-T结合蛋白的蛋白质已从酵母中纯化出来，它能特异性地结合酵母自主复制序列（ARS）元件共有核心序列的富含T的链。从凝胶迁移率变动实验检测可知，ssARS-T蛋白表现出序列特异性单链DNA结合蛋白的特征。ARS核心序列的互补富含A的链结合较弱，且未检测到核心序列双链形式的结合。已知在体内消除ARS功能的核心序列中的三个单碱基替换，在体外也导致核心序列与ssARS-T蛋白的结合变弱。体外突变序列的结合与这些序列在体内的ARS特性之间的强相关性表明，ssARS-T蛋白在酵母ARS元件复制起始过程中具有重要功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b3/452742/87f5764d26b7/emboj00102-0248-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b3/452742/61d5e0ef03de/emboj00102-0246-a.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b3/452742/87f5764d26b7/emboj00102-0248-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b3/452742/61d5e0ef03de/emboj00102-0246-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b3/452742/2dcfed5b2593/emboj00102-0246-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b3/452742/0ca8c3a399b0/emboj00102-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b3/452742/3697001f482e/emboj00102-0247-b.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b3/452742/4ffe2241f58a/emboj00102-0248-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e6b3/452742/87f5764d26b7/emboj00102-0248-b.jpg

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本文引用的文献

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Identification of silencer binding proteins from yeast: possible roles in SIR control and DNA replication.从酵母中鉴定沉默子结合蛋白：在SIR调控和DNA复制中的可能作用。
EMBO J. 1987 Feb;6(2):461-7. doi: 10.1002/j.1460-2075.1987.tb04776.x.
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DNA sequences required for the in vitro replication of adenovirus DNA.腺病毒DNA体外复制所需的DNA序列。
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Deletion mutations affecting autonomously replicating sequence ARS1 of Saccharomyces cerevisiae.影响酿酒酵母自主复制序列ARS1的缺失突变
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Identification of DNA-binding proteins that recognize a conserved type I repeat sequence in the replication origin region of Tetrahymena rDNA.鉴定在四膜虫核糖体DNA复制起始区域识别保守的I型重复序列的DNA结合蛋白。
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