Palzkill T G, Oliver S G, Newlon C S
Nucleic Acids Res. 1986 Aug 11;14(15):6247-64. doi: 10.1093/nar/14.15.6247.
Four fragments of Saccharomyces cerevisiae chromosome III DNA which carry ARS elements have been sequenced. Each fragment contains multiple copies of sequences that have at least 10 out of 11 bases of homology to a previously reported 11 bp core consensus sequence. A survey of these new ARS sequences and previously reported sequences revealed the presence of an additional 11 bp conserved element located on the 3' side of the T-rich strand of the core consensus. Subcloning analysis as well as deletion and transposon insertion mutagenesis of ARS fragments support a role for 3' conserved sequence in promoting ARS activity.
已对携带自主复制序列（ARS）元件的酿酒酵母III号染色体DNA的四个片段进行了测序。每个片段都包含多个序列拷贝，这些序列与先前报道的11 bp核心共有序列至少有11个碱基中的10个具有同源性。对这些新的ARS序列和先前报道的序列进行的调查显示，在核心共有序列富含T链的3'侧存在一个额外的11 bp保守元件。ARS片段的亚克隆分析以及缺失和转座子插入诱变支持3'保守序列在促进ARS活性中的作用。
