Bisaillon J G
Rev Can Biol. 1977 Sep;36(3):283-9.
We have produced pertussis vaccines with laboratory and industrial methods. The characteristic of laboratory cultivation of microorganisms is, in this context, growth on Hornibrook medium in low form flask and in stationary culture. Industrial cultivation is done in homogenous culture on a B-2 medium in fermentor. The strains utilized were isolated from whooping-cough cases in the Montreal region. The yield (org. x 10(9)/ml) obtained with an industrial cultivation of B. pertussis was 4 to 7 times higher than that reached with a laboratory cultivation of this microorganism. The non-toxicity as expressed in weight gain of mice was shown for both types of vaccine. The vaccines produced in fermentor were less histamino sensibilizing for mice than the one produced in stationary flash culture. The quality of the vaccines achieved by industrial method is easily reproducible due to the fact that enough variables can be measured.
我们已经用实验室方法和工业方法生产了百日咳疫苗。在此背景下，微生物实验室培养的特点是在低型烧瓶中的霍尼布鲁克培养基上进行静止培养。工业培养是在发酵罐中于B - 2培养基上进行均匀培养。所使用的菌株是从蒙特利尔地区的百日咳病例中分离出来的。百日咳杆菌工业培养获得的产量（有机物×10⁹/毫升）比该微生物实验室培养的产量高4至7倍。两种疫苗对小鼠体重增加所表现出的无毒特性均得到验证。与在静止烧瓶培养中生产的疫苗相比，发酵罐中生产的疫苗对小鼠的组胺致敏性更低。由于可以测量足够多的变量，通过工业方法生产的疫苗质量易于重现。
