Department of Chemistry, Cottingham Rd, Hull, UK HU6 7RX.
Analyst. 2010 Feb;135(2):302-5. doi: 10.1039/b917643h. Epub 2009 Nov 30.
A novel method to determine nitric oxide (NO) in biological tissue samples with minimal interference from the cellular detritus is described. Methylpiperazinobenzenediamine, consisting of an o-phenylenediamine and a methyl piperazine group, was chosen as a probe for the detection of NO by mass spectrometry (MS) in biological tissue samples. The o-phenylenediamine group reacts with NO to form a characteristic benzotriazole. The product was identified using electrospray ionization mass spectrometry (ESI-MS) and the method validated within the range of 95-1900 nM. NO levels associated with tissue biopsies (approximately 10 mg) from rat vasculature and intestine tissue biopsies have been successfully determined. The different rates of NO generated from tissue samples under hypoxic and normoxic conditions have been studied by this simple and sensitive method.
本文描述了一种从生物组织样品中测定痕量一氧化氮（NO）的新方法，该方法可最大限度地减少细胞碎片的干扰。选择包含邻苯二胺和甲基哌嗪基团的甲哌啶苯二胺作为探测生物组织样品中 NO 的质谱（MS）探针。邻苯二胺基团与 NO 反应生成特征苯并三唑。通过电喷雾电离质谱（ESI-MS）鉴定产物，并在 95-1900 nM 范围内验证该方法。成功地测定了来自大鼠血管和肠组织活检的与组织活检相关的 NO 水平。通过这种简单而灵敏的方法研究了缺氧和正常氧条件下组织样品产生的 NO 速率的不同。
