Institute of Clinical Pharmacology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
Analyst. 2011 Jan 21;136(2):407-10; discussion 411. doi: 10.1039/c0an00411a. Epub 2010 Oct 8.
Recently, Shen et al. (Analyst, 2010, 135, 302) reported on a flow injection analysis (FIA) ESI-MS/MS approach for the determination of the short-lived gaseous nitric oxide (NO) in biological samples. This method is based on the reaction of NO, and presumably of other NO-derived oxides such as N(2)O(3), with the vicinal amino groups of methylpiperazinobenzendiamine to form a benzotriazole derivative. Under MS/MS conditions, the protonated derivative loses molecular nitrogen (N(2)) from the triazole ring and the product ion formed is utilized for quantitative analyses. This seems to be the first ESI-MS/MS method for authentic NO detection and quantification. However, the ESI-MS/MS method reported by Shen et al. deserves some critical discussion.
最近，沈等人（分析家，2010 年，135，302）报道了一种用于测定生物样品中短寿命气态一氧化氮（NO）的流动注射分析（FIA）ESI-MS/MS 方法。该方法基于 NO 与甲基哌嗪苯二胺的顺式氨基反应，形成苯并三唑衍生物。在 MS/MS 条件下，质子化衍生物从三唑环中失去分子氮（N(2)），形成的产物离子用于定量分析。这似乎是第一个用于真实 NO 检测和定量的 ESI-MS/MS 方法。然而，沈等人报道的 ESI-MS/MS 方法值得一些批判性讨论。
