Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China.
Analyst. 2010 Feb;135(2):332-6. doi: 10.1039/b921167e. Epub 2009 Dec 11.
A sensitive DNA biosensor based on dual-amplification of thrombin and thiocyanuric acid-gold nanoparticle (TCA-AuNP) network is developed. First, the sandwich hybridization is formed by the capture probe immobilized on the surface of magnetic beads (MBs), the target DNA and the reporter probe loaded on PbS nanoparticles (PbS NPs). The PbS NPs contain two kinds of DNA sequences, one is the reporter probe complementary to the target DNA, and the other is the thrombin aptamer I. Through the specific recognition for thrombin, thrombin aptamer II labeled gold nanoparticles are linked to the sandwich complex, and further fabricate a network with TCA. AuNPs are released and dissolved into Au(3+), which catalyzes luminol chemiluminescence (CL) reaction. Due to the dual-amplification effects of thrombin-labeled PbS NPs and the TCA-AuNP network, a significant sensitivity enhancement of this DNA biosensor could be obtained, in the range of 2.0 x 10(-16) M to 3.5 x 10(-14) M, with a limit of detection (LOD) as low as 1.0 x 10(-16) M.
基于凝血酶和硫氰酸-金纳米粒子（TCA-AuNP）网络的双重放大作用，开发了一种灵敏的 DNA 生物传感器。首先，通过固定在磁性珠（MBs）表面的捕获探针、目标 DNA 和负载在 PbS 纳米粒子（PbS NPs）上的报告探针形成三明治杂交体。PbS NPs 包含两种 DNA 序列，一种是与目标 DNA 互补的报告探针，另一种是凝血酶适体 I。通过对凝血酶的特异性识别，凝血酶标记的金纳米粒子与三明治复合物相连，并进一步与 TCA 形成网络。AuNPs 被释放并溶解成 Au(3+)，其催化鲁米诺化学发光（CL）反应。由于凝血酶标记的 PbS NPs 和 TCA-AuNP 网络的双重放大效应，该 DNA 生物传感器在 2.0 x 10(-16) M 至 3.5 x 10(-14) M 的范围内具有显著的灵敏度增强，检测限（LOD）低至 1.0 x 10(-16) M。
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